Functional Similarities of Recombinant OLP and Cytokinin-Binding Protein 2(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
CBP1 and CBP2 are cytokinin-binding proteins isolated from tobacco callus. In particularly, CBP2 is a 26-kDa protein with high affinity (Kd = 1.08×10^<-6>M) for cytokinin [Kobayashi et al. Plant Cell Physiol. 41(2) : 148-157 (2000)] and the N-terminal amino acid analysis of CBP2 showed high sequence homology (92.9%) to tobacco osmotin-like protein (OLP). To compare the properties of OLP and CBP2, recombinant OLP was purified, and binding to benzyladenine (BA) was examined. The inclusion bodies of recombinant OLP were solubilized in 8 M urea and purified on an SP-Sepharose column. SDS-PAGE analysis of the purified recombinant OLP revealed a single band of 26 kDa. The Kd of solublized recombinant OLP to BA obtained from a Scachard plot was 1.10×10^<-6>M, which was similar to the Kd of CBP2 to BA (1.08×10^<-6> M).
- 社団法人日本農芸化学会の論文
- 2001-12-23
著者
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Sato Fumihiko
Department of Medicine, Metabolism and Endocrinology, Juntendo University School of Medicine
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Sato F
Div. Of Integrated Life Sci. Graduate School Of Biostudies Kyoto Univ.
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Sato Fumihiko
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Sato F
Division Of Integrated Life Science Graduate School Of Biostudies Kyoto University
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Koiwa Hisashi
Department Of Applied Life Sciences Kyoto University
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Kobayashi K
Division Of Microbiology National Institute Of Health Sciences
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IGARASHI Daisuke
Laboratory of Life Sciences, Tokyo Gakugei University
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ITO Noboru
Laboratory of Life Sciences, Tokyo Gakugei University
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HARADA Kazuo
Laboratory of Life Sciences, Tokyo Gakugei University
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KOBAYASHI Koh
Laboratory of Life Sciences, Tokyo Gakugei University
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Sato Fumihiko
Graduate School Of Biostudies Kyoto University
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Ito N
Laboratory Of Life Sciences Tokyo Gakugei University
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Kobayashi Koh
Laboratory Of Life Sciences Tokyo Gakugei University
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KATO Hiroaki
RIKEN Harima Inst.
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Igarashi D
Laboratory Of Life Sciences Tokyo Gakugei University
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Harada Kazuo
Laboratory Of Life Sciences Tokyo Gakugei University
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Sato Fumihiko
Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University
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