Purification and Characterization of Tobacco Pathogenesis-Related Protein PR-5d, an Antifungal Thaumatin-like Protein

スポンサーリンク

概要

• 論文の詳細を見る

• Cultured tobacco cells accumulate several pathogenesis-related proteins. A neutral PR-5 protein, PR-5d, was purified to homogeneity from such cells. PR-5d has highly hydrophobic characteristics, but hydropathy analysis of its primary structure did not show a hydrophobic domain. ID a series of bioassays, purffied PR-5d showed inhibitory activity against several phytopathogenic and non-phyiopathogenic fungi as do other members of the PR-5 protein family. To study the antifungal mechanism based on three dimensional structure of PR-5d, purified PR-5d was crystallized. The prelinrinary X-ray analysis of the crystal revealed that the crystals belong to space group C2, with cell dimensions a=80.2 Å, b =63.8 Å, c=45.7 Å, and β= 107.2゜, and diffract at least 1.8 A resolution.

• 日本植物生理学会の論文

著者

• Sato Fumihiko

  Department of Medicine, Metabolism and Endocrinology, Juntendo University School of Medicine

• Yamada Y

  Doshisha Women's Coll. Liberal Arts Kyoto Jpn

• Yamada Y

  The Department Of Mechanical Engineering And Intelligent Systems University Of Electro-communication

• Sato Fumihiko

  Grad. Sch. Agri. Kyoto Univ.:grad. Sch. Biostudies Kyoto Univ.

• Sato Fumihiko

  Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University

• KATO Hiroaki

  Institute for Chemical Research, Kyoto University

• Yamada Yukio

  Fine Chemicals Research Laboratories Kaneka Corporation

• Yamada Yasuyuki

  Graduate School Of Biological Science Nara Institute Of Science And Technoiogy

• Yamada Yukio

  Mechanical Engineering Laboratory Material Science And Bioengineering Department Aist

• Yamada Y

  Fac. Of Education Utsunomiya Univ.

• Oda Junichi

  Institute For Chemical Research Kyoto University

• Koiwa Hisashi

  Department Of Applied Life Sciences Kyoto University

• Yamada Y

  Faculty Of Education Utsunomiya University

• Nakatsu Toru

  Institute for chemical Research, Kyoto University

• Nakatsu Toru

  Riken Harima Inst.

• KATO Hiroaki

  RIKEN Harima Inst.

• Kato Hiroaki

  Institute For Chemical Research Kyoto University

• Yamada Yuko

  Kyoto Univ. Kyoto Jpn

関連論文

• CYCLIC ELECTRON TRANSPORT MEDIATED BY NAD(P)H DEHYDROGENASE IN TOBACCO CHLOROPLASTS
• Intracellular lipid accumulation and insulin resistance in skeletal muscle and liver
• Therapeutic Efficacy of Mitiglinide Combined with Once Daily Insulin Glargine after Switching from Multiple Daily Insulin Regimen of Aspart Insulin and Glargine in Patients with Type 2 Diabetes Mellitus
• Tobacco ndhC-K-J disruptants created by chloroplast transformation and their characterization
• The Aravidopsis high Fo mutant impaired in 33kDa protein in oxygen evolving complex (OEC)
• INHIBITION OF PHOTOSYNTHETIC ELECTRON TRANSPORT IN ATRAZINE-RESISTANT TOBACCO CELLS BY LICHEN ACIDS
• Fabrication of Realistic and Dynamic Human Head Phantoms
• Isolation of a Protease-Deficient Mutant of Bacillus brevis and Efficient Secretion of a Fungal Protein Disulfied Isomerase by the Mutant
• Simple and Rapid Screening Method for Photosystem II Inhibitory Herbicides Using Photoautotrophically Cultured Plant Cells with Chlorophyll Fluorescence Monitoring
• A Potent Hypotensive Peptide, Novokinin, Induces Relaxation by AT2- and IP-Receptor-Dependent Mechanism in the Mesenteric Artery from SHRs
• Design of a Highly Potent Anti-hypertensive Peptide Based on Ovokinin(2-7)(Biochemistry & Molecular Biology)
• Designing Potent Derivatives of Ovokinin(2-7), an Anti-hypertensive Peptide Derived from Ovalbumin
• Application of the Freeze-Blast Method to Disruption of Cultured Plant Cells
• Overexpression of Glutathione Synthetase in Escherichia coli(Biological Chemistry)
• Bacterial Production and Purification of Phosphorylatable Phosphoenolpyruvate Carboxylase from Tobacco
• Purification and Characterization of Phosphoenolpyruvate Carboxylase of Photomixotrophically Cultured Green Tobacco Cells
• Production of Thermotolerant N-Carbamyl-D-Amino Acid Amidohydrolase by Recombinant Escherichia coli
• Thermostability Reinforcement through a Combination of Thermostability-related Mutations of N-Carbamyl-D-Amino Acid Amidohydrolase
• Immobilization of N-Carbamyl-D-Amino Acid Amidohydrolase
• Relationship Between an Increase in Thermostability and Amino Acid Substitutions in N-Carbamyl-D-Amino Acid Amidohydrolase
• Increase in Thermostability of N-Carbamyl-D-Amino Acid Amidohydrolase on Amino Acid Substitutions
• Screening, Characterization, and Cloning of the Gene for N-Carbamyl-D-Amino acid Amidohydrolase from Thermotolerant Soil Bacteria
• Isolation of Agrobacterium sp. Strain KNK712 That Produces N-Carbamyl-D-Amino Acid Amidohydrolase, Cloning of the Gene for this Enzyme, and Properties of the Enzyme
• Strict Glycemic Control Ameliorates the Increase of Carotid IMT in Patients with Type 2 Diabetes
• Molecular Cloning of a Fungal cDNA Encoding Protein Disulfide Isomerase
• Purification of Protein Disulfide Isomerase from a Thermophilic Fungus
• CRYSTALLIZATION OF TOBACCO OSMOTIN-LIKE PROTEIN.
• High-Field Magnetization Study of Sm_2Fe_N_x and Related Compounds
• Magnetization Curves of the Reentrant-Spin-Glass Fe_Mn_TiO_3 : Dependence on the Sweep Rate of Magnetic Fields(Magnetism)
• High-Field Magnetization of Some Mn Alloys(Magnetism)
• High-Field Magnetization Process in R_2Fe_B and R_2Fe_N_x Compounds(Magnetism)
• Magnetization Anomaly of Sm_2fe_14B Single Crystal in High Magnetic Fields
• Molecular Properties and Activity of Amino-Terminal Truncated Forms of Lipase Activator Protein
• Calcium Ion-Dependent Reactivation of a Pseudomonas Lipase by Its Specific Modulating Protein, LipB^1
• DEFECT IN CHLOROPLASTIC NAD (P) H DEHYDROGENASE COMPLEX RESULTED IN STROMAL OVER-REDUCTION
• Development of a Simple Non-Freezing Method to Preserve Cultured Plant Cells
• Pigment Synthesis by Immobilized Cultured Cells of Lavandula vera and Characterization of a Component of the Pigments(Microbiology & Fermentation Industry)
• Influence of Carbon Source on Pigment Production by Immobilized Cultured Cells of Lavandula vera
• Immobilized Plant Cell Reactor for Continuous Production of Blue Pigments(Microbiology & Fermentation Industry)
• Enhancement of Pigment Productivity of Immobilized Cultured Lavandula vera Cells by Limitation of Nitrogen Sources
• Phylogenetic Relationships of Species of the Genus Saccharomyces MEYEN ex REESS Deduced from Partial Base Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae)
• Phylogenetic Relationships of Species of the Genus Kluyveromyces VAN DER WALT (Saccharomycetaceae) Deduced from the Partial Sequences of 18S and 26S Ribosomal RNAs
• Molecular Cloning and Characterization of S-Adenosyl-L-Methionine : Scoulerine-9-O-Methyltransferase from Cultured Cells of Coptis japonica : GENE STRUCTURE AND EXPRESSION : PROTEINS, ENZYMES AND METABOLISM
• Analysis of Nucleoid-Proteins in Tobacco Chloroplasts
• Photoautotrophic and Photomixotrophic Culture of Green Tobacco Cells in a Jar-Fermenter
• An efficient method of selecting photoautotropbic cells from cultured heterogeneous cells
• 葉緑体NAD(P)Hデヒドロゲナーゼ複合体変異株での電子伝達活性
• Overexpression and Purification of Asparagine Synthetase from Escherichia coli
• Genomic Structure and Promoter Analysis of Phosphoenolpyruvate Carboxylase in a C3 Plant, Nicotiana sylvestris(Biochemistry & Molecular Biology)
• Insight into the Molecular Evolution of Two Tropinone Reductases
• Structures and Expression Patterns of Two Tropinone Reductase Genes from Hyoscyamus niger
• Purification and Characterization of Tobacco Pathogenesis-Related Protein PR-5d, an Antifungal Thaumatin-like Protein
• Spasmolytic Activity of Aurapten Analogs
• The Phylogeny of Species of the Genus Issatchehkia KUDRIAVZEZV (Saccharomycetaceae) Based on the Partial Sequences of 18S and 26S Ribosomal RNAs
• Effects of Oxygen on Nicotine and Tropane Alkaloid Production in Cultured Roots of Duboisia myoporoides
• Scopolamine Yield in Cultured Roots of Duboisia myoporoides Improved by a Novel Two-stage Culture Method
• Increase of Scopolamine Production by High Density Culture of Duboisia myoporoides Roots
• Tropane Alkaloid Production in Root Cultures of Duboisia myoporoides Obtained by Repeated Selection
• Time-Resolved Measurements of in vivo Optical Properties of Piglet Brain
• Anatomical and Functional Images of in vitro and in vivo Tissues by NIR Time-domain Diffuse Optical Tomography(Reviewed Papers Accepted for Publication in this Special Issue)(Special Issue on Bioengineering)
• Time-Resolved Diffuse Optical Tomography Using a Modified Generalized Pulse Spectrum Technique(Special Issue on Measurements and Visualization Technology of Biological Information)
• Transgenic Tobacco Plants with Two Consecutive Oxidation Reactions Catalyzed by Hyoscyamine 6β-Hydroxylase
• Functional Similarities of Recombinant OLP and Cytokinin-Binding Protein 2(Biochemistry & Molecular Biology)
• Ethylene-Induced Gene Expression of Osmotin-Like Protein, a Neutral Isoform of Tobacco PR-5, is Mediated by the AGCCGCC cis-Sequence
• Studies of Diluted Antiferromagnet Fe_Mg_TiO_3 : Percolation Concentration and Cluster-Glass
• Analysis of antifungal activity andlocalization of tobacco osmotin-like protein.
• ROLE OF N-TERMINAL 18 AMINO-ACID RESIDUES OF OEC23 IN PS II FOR ION RETENTION
• Characterization of Accumulation of Tobacco PR-5 Proteins by IEF-Immunoblot Analysis : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
• The Phylogeny of Williopsis salicorniae HINZELIN, KURTZMAN et SMITH Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae)
• Accumulation of the Bioactive Peptides, Novokinin, LPYPR and Rubiscolin, in Seeds of Genetically Modified Soybean
• A Potent Hypotensive Peptide, Novokinin, Induces Relaxation by AT_2- and IP-Receptor-Dependent Mechanism in the Mesenteric Artery from SHRs
• Isolation of Putative Glycoprotein Gene from Early Somatic Embryo of Carrot and its Possible Involvement in Somatic Embryo Development
• CONCENTRATION OF LEVOFLOXACIN IN CERVICAL MUCUS AND ITS CLINICAL EFFECTS ON CERVICITIS
• Photosynthetic carbon metabolism in photoautotrophically and photomixotrophically cultured tobacco cells
• Photosynthetic Characteristics and Carbonic Anhydrase Activity in Cells Cultured Photoautotrophically and Mixotrophically and Cells Isolated from Leaves
• Cloning and Nucleotide Sequence of ApaLI Restriction-modification System from Acetobacter pasteurianus IFO 13753
• Cloning and Nucleotide Sequence of the AgeI Methylase Gene from Agrobacterium gelatinovorum IAM 12617, a Marine Bacterium
• Soymorphin-5, a soy-derived μ opioid peptide, improves glucose and lipid metabolism in diabetic KK-Ay mice
• The Phylogeny of Species of the Genus Saccharomycopsis SCHIONNING (Saccharomycetaceae) Based on the Partial Sequences of 18S and 26S Ribosomal RNAs
• Should Petasospora BOIDIN et ABADIE (Saccharomycetaceae) Be Retained? : The Phylogeny Based on the Partial Sequences of 18S and 26S Ribosomal RNAs
• The Phylogeny of Yamadazyma ohmeri (ETCHELLS et BELL) BILLON-GRAND Based on the Partial Sequences of 18S and 26S Ribosomal RNAs : The Proposal of Kodamaea Gen. Nov. (Saccharomycetaceae)
• The Phylogenetic Relationships of Pichia jadinii, Formerly Classified in the Genus Hansenula, and Related Species Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae)
• The Phylogenetic Relationships of the Q_9-equipped, Hat-shaped Ascospore-forming Species of the Genus Yamadazyma BILLON-GRAND (Saccharomycetaceae) Based on the Partial Sequences of 18S and 26S Ribosomal RNAs
• The Phylogenetic Relationships of Methanol-assimilating Yeasts Based on the Partial Sequences of 18S and 26S Ribosomal RNAs : The Proposal of Komagataella Gen. Nov.(Saccharomycetaceae)
• The Phylogenetic Relationships of Species of the Genus Dekkera VAN DER WALT Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae)
• Microbial Transformation of (±)-Epoxyaurpten by Pseudomonas aeruginosa
• Adopting Due-Date Buffer for Real-Time Scheduling Using Lagrangean Relaxation Method in an APS Environment
• 6B2 ADOPTING DUE-DATE BUFFER FOR REAL-TIME SCHEDULING USING LAGRANGEAN RELAXATION METHOD IN AN APS ENVIRONMENT(Technical session 6B : Lagrangian decomposition)
• Photoautotrophy and the photosynthetic potential of chlorophyllous cells in mixotrophic cultures
• Changes Related to Salt Tolerance in Thylakoid Membranes of Photoautotrophically Cultured Green Tobacco Cells : STRUCTURE AND FUNCTION OF CELLS
• The Phylogenetic Relationships of the Saturn-shaped Ascospore-forming Species of the Genus Williopsis Zender and Related Genera Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae) : The Proposal of Komagataea Gen.Nov.
• The Phylogenetic Relationships of the Hat-shaped Ascospore-forming, Nitrate-assimilat-ing Pichia Species, Formerly Classified in the Genus Hansenula SYDOW et SYDOW,Based on the Partial Sequences of 18S and 26S Ribosomal RNAs (Saccharomycetaceae) : The Pro
• The Phylogenetic Relationships of Rhodosporidium dacryoidum FELL, HUNTER et TALLMAN Based on the Partial Sequences of 18S and 26S Ribosomal RNAs : The Proposal of Sakaguchia Gen. Nov., a Heterobasidiomycetous Yeast Genus
• The photoautotrophic culture of chlorophyllous cells
• An in Vitro Analysis System Using a Fluorescence Protein Reporter for Evaluating Anti-Inflammatory Effects in Macrophages
• Isoquinoline Alkaloid Biosynthesis is Regulated by a Unique bHLH-Type Transcription Factor in Coptis japonica
• 2L1524 光合成酸素発生系における表在性タンパク質PsbPの相互作用と機能(光生物_光合成,第49回日本生物物理学会年会)
• A case of long-standing autoimmune type 1 diabetes with common variable immunodeficiency
• An Improved Medium for the Photoautotrophic Culture of Cytisus scoparius Link Cells
• Characterization of chromosomal aberrations in thymic MALT lymphoma

もっと見る 閉じる

スポンサーリンク