Chromosomal Transformation in Saccharomyces cerevisiae with DNA Isolated by Pulse Field Gel Electrophoresis(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
We isolated and purified yeast chromosome DNA molecules using pulse field gel electrophoresis (PFG). The isolated DNA had nearly the same size as the native chromosomal DNA on PFG. We could directly transform Saccharomyces cerevisiae yeasts with it, and obtain transformants that were selected by complementation of several markers. They had new chromosome DNA bands observed on PFG. The new chromosome was very stable during mitosis and mating processes, and each of the three homologous chromosomes in the derivative zygotes of transformants was separated equally in daughter cells.
- 社団法人日本農芸化学会の論文
- 1990-06-23
著者
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Obata Takaji
National Research Institute of Brewing
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Tamura Gakuzo
Research Institute Of Brewing Resources Co. Ltd.
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GOTO Kuniyasu
National Research Institute of Brewing
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HARA Shodo
National Research Institute of Brewing
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MOTOYOSHI Tohru
Research Institute of Brewing Resources Co., Ltd.
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Motoyoshi Tohru
Research Institute Of Brewing Resources Co. Ltd.
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Hara S
National Research Institute Of Brewing
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Goto K
Osaka Inst. Technology Osaka Jpn
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Obata T
National Research Institute Of Brewing
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Goto Kuniyasu
National Res. Inst. Of Brewing
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TAMURA Gakuzo
Research Institute of Brewing Resources Co., Ltd.
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