Cloning and Nucleotide Sequence of the KHR Killer Gene of Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
The KHR gene cloned from a genomic library was on 4.7-kbp DNA fragment and was inserted into YCpGll vector (KHR-YCp) and YEp vector (KHP-YEp). Transformants with KHR-YEp could secrete 3-4 times as much killer toxin into the media as the donor strain. The complete nucleotide sequence of the KHR gene was analyzed. It was found that the KHR gene consisted of 888 bp. It was suggested that this protein was processed before being secreted into the media, because its molecular mass presumed from the nucleotide sequence was larger than that of the mature killer toxin.
- 社団法人日本農芸化学会の論文
- 1990-04-23
著者
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Obata Takaji
National Research Institute of Brewing
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GOTO Kuniyasu
National Research Institute of Brewing
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HARA Shodo
National Research Institute of Brewing
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Hara S
National Research Institute Of Brewing
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Goto K
Osaka Inst. Technology Osaka Jpn
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Obata T
National Research Institute Of Brewing
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IWATUKI Yasuhiro
National Research Institute of Brewing
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KITANO Kazuyosi
National Research Institute of Brewing
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Goto Kuniyasu
National Res. Inst. Of Brewing
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