Isolation and Properties of a Chromosome-dependent KHR Killer Toxin in Saccharomyces cerevisiae(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
A strain of the yeast Saccharomyces cerevisiae coding for KHR on the chromosome secreted a toxin that kills sensitive yeasts. The transformants of multicopy vectors carrying the KHR gene could secrete 3-4-fold the killer toxin of the donor strain. This toxic substance was purified 80-fold in specific activity from the culture filtrate by gel filtration and hydrophobic column chromatography. The purified toxin gave a single protein band with molecular mass of 20kDa on SDS-PAGE and had an isoelectric point of pH 5.3. The toxin had novel killer activity against Candida glabrata and S. cerevisiae, but did not affect bacteria, fungi, or other yeasts.
- 社団法人日本農芸化学会の論文
- 1990-02-23
著者
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Obata Takaji
National Research Institute of Brewing
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GOTO Kuniyasu
National Research Institute of Brewing
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HARA Shodo
National Research Institute of Brewing
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Hara S
National Research Institute Of Brewing
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Goto K
Osaka Inst. Technology Osaka Jpn
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Obata T
National Research Institute Of Brewing
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KITANO Kazuyosi
National Research Institute of Brewing
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IWASE Tosinori
National Research Institute of Brewing
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KICHISE Kouji
National Research Institute of Brewing
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TOTUKA Akira
National Research Institute of Brewing
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Goto Kuniyasu
National Res. Inst. Of Brewing
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