Gene Organization for Nitric Oxide Reduction in Alcaligenes faecalis S-6
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概要
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norB and norC encoding the cytochrome b-containing subunit and the cytochrome c-containing subunit, respectively, of the nitric oxide reductase (NOR) in Alcaligenes faecalis S-6 were cloned and sequenced. Both NorB and NorC showed more than 40% sequence identity to the corresponding subunits of cytochrome bctype NORs in other denitrifying bacteria. norCB was in a gene cluster containing seven other genes ; these were named dnr, orf2,orf3,norE, norF, norQ, and norD on the basis of their similarity with NOR systems in other bacteria. Potential FNR-binding sites were present in front of norCB, norEF, and/or orf2/orf3,suggesting that most of these genes are regulated simultaneously by an FNR-related protein. NorB and NorC proteins produced in the membrane fraction in Escherichia coli showed no enzyme activity, probably due to lack of NorQ and NorD, which appear to perform some essential function for activation of the NorB-NorC complex in the recombinant E. coli.
- 2000-04-23
著者
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NISHIYAMA Makoto
Biotechnology Research Center, The University of Tokyo
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Nishiyama Makoto
Biotechnology Research Center The University Of Tokyo
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Horinouchi Sueharu
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Tanokura Masaru
Biotechnology Research Center The University Of Tokyo
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KUKIMOTO Mutsuko
Departmentof Biotechnology, The University of Tokyo
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Kukimoto Mutsuko
Departmentof Biotechnology The University Of Tokyo
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