Production of Novel Antioxidative Prenyl Naphthalen-ols by Combinational Bioconversion with Dioxygenase PhnA1A2A3A4 and Prenyltransferase NphB or SCO7190

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概要

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• We performed combinational bioconversion of substituted naphthalenes with PhnA1A2A3A4 (an aromatic dihydroxylating dioxygenase from marine bacterium Cycloclasticus sp. strain A5) and prenyltransferase NphB (geranyltransferase from Streptomyces sp. strain CL190) or SCO7190 (dimethylallyltransferase from Streptomyces coelicolor A3(2)) to produce prenyl naphthalen-ols. Using 2-methylnaphthalene, 1-methoxynaphthalene, and 1-ethoxynaphthalene as the starting substrates, 10 novel prenyl naphthalen-ols were produced by combinational bioconversion. These novel prenyl naphthalen-ols each showed potent antioxidative activity against a rat brain homogenate model. 2-(2,3-Dihydroxyphenyl)-5,7-dihydroxy-chromen-4-one (2′,3′-dihydroxychrysin) generated with another aromatic dihydroxylating dioxygenase and subsequent dehydrogenase was also geranylated at the C-5′-carbon by the action of NphB.

著者

• SHINDO Kazutoshi

  Department of Food and Nutrition, Japan Women's University

• TANAKA Ayumi

  Department of Materials Science and Engineering, Faculty of Engineering and Resource Science, Akita

• NISHIYAMA Makoto

  Biotechnology Research Center, The University of Tokyo

• KUZUYAMA Tomohisa

  Biotechnology Research Center, The University of Tokyo

• TACHIBANA Ayako

  Department of Food and Nutrition, Japan Women’s University

• TOBA Shizuka

  Department of Food and Nutrition, Japan Women’s University

• YUKI Emi

  Department of Food and Nutrition, Japan Women’s University

• OZAKI Taro

  Biotechnology Research Center, The University of Tokyo

• KUMANO Takuto

  Biotechnology Research Center, The University of Tokyo

• MISAWA Norihiko

  Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University

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