Comparison of Two Vectors for Functional Expression of a Bacterial Cytochrome P450 Gene in Escherichia coli Using CYP153 Genes
スポンサーリンク
概要
- 論文の詳細を見る
Two vectors, pT7NScamAB and pRED, have been used for the functional expression of bacterial class I cytochrome P450 (P450) genes in Escherichia coli, which utilize putidaredoxin reductase (CamA) and putidaredoxin (CamB), and the reductase domain of a self-sufficient P450RhF respectively, for electron transfer from NAD(P)H to a P450 protein. We here compared the efficiency of bioconversion with the two vectors towards n-octane, cyclohexane, n-butylbenzene, and 2-n-butylbenzofuran using two well-characterized CYP153A genes, aciA and CYP153A13a (P450balk). As for n-octane bioconversion, aciA and pT7camAB was the best combination for the production of 1-octanol and 1,8-octanediol. As for the bioconversion of cyclohexane, n-butylbenzene and 2-n-butylbenzofuran, CYP153A13a with pRED achieved the most efficient bioconversion, as compared by conversion ratio per active CYP153A protein content. It was also found that 2-n-butylbenzofuran is biotransformed into 4-benzofuran-2-yl-butyric acid via 4-benzofuran-2-yl-butan-1-ol with E. coli cells expressing CYP153A.
- 社団法人 日本農芸化学会の論文
著者
-
SHINDO Kazutoshi
Department of Food and Nutrition, Japan Women's University
-
ARISAWA Akira
Bioresource Laboratories, Mercian Corp.
-
Misawa Norihiko
Marine Biotechnology Institute Co., Ltd.
-
Fujita Naoya
Marine Biotechnology Institute
-
Sumisa Futoshi
Marine Biotechnology Institute
-
KABUMOTO Hiroki
Bioresource Laboratories, Mercian Corporation
-
IKENAGA Hiroshi
Marine Biotechnology Institute
関連論文
- Methyl Glucosyl-3,4-dehydro-apo-8'-lycopenoate, a Novel Antioxidative Glyco-C_-carotenoic Acid Produced by a Marine Bacterium Planococcus maritimus
- Generation of New Benanomicin Analogues by Biotransformation Using Escherchia coli Expressing Actinomycete Cytochrome P450
- Enrichment of Carotenoids in Flaxseed(Linum usitatissimum) by Metabolic Engineering with Introduction of Bacterial Phytoene Synthase Gene crtB(PLANT BIOTECHNOLOGY)
- Gerfelin, a Novel Inhibitor of Geranylgeranyl Diphosphate Synthase from Beauveria felina QN22047 : I. Taxonomy, Fermentation, Isolation, and Biological Activities
- Comparison of Two Vectors for Functional Expression of a Bacterial Cytochrome P450 Gene in Escherichia coli Using CYP153 Genes
- Analysis of Genes Dominantly Expressed in Rat Cerebral Endothelial Cells Using Suppression Subtractive Hybridization
- Glutathione Reacts with Glyoxal at the N-Terminal
- Production of Novel Antioxidative Prenyl Naphthalen-ols by Combinational Bioconversion with Dioxygenase PhnA1A2A3A4 and Prenyltransferase NphB or SCO7190
- A Novel Diol-derivative of Chalcone Produced by Bioconversion, 3-(2,3-Dihydroxyphenyl)-1-phenylpropan-1-one, Activates Phosphorylation of ERK and CREB in Cultured Rat Cortical Neurons
- Isolation and Functional Analysis of Cytochrome P450 CYP153A Genes from Various Environments
- Protein Engineering on Biphenyl Dioxygenase for Conferring Activity to Convert 7-Hydroxyflavone and 5,7-Dihydroxyflavone (Chrysin)(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Production of α,ω-Alkanediols Using Escherichia coli Expressing a Cytochrome P450 from Acinetobacter sp. OC4
- Gerfelin, a Novel Inhibitor of Geranylgeranyl Diphosphate Synthase from Beauveria felina QN22047 : II. Structural Elucidation
- Hydroxylation of Testosterone by Bacterial Cytochromes P450 Using the Escherichia coli Expression System
- Characterization of Cyanobacterial Carotenoid Ketolase CrtW and Hydroxylase CrtR by Complementation Analysis in Escherichia coli
- Characterization and Antioxidative Activities of Rare C50 Carotenoids-Sarcinaxanthin, Sarcinaxanthin Monoglucoside, and Sarcinaxanthin Diglucoside-Obtained from Micrococcus yunnanensis
- SAR Study of a Novel Triene-ansamycin Group Compound, Quinotrierixin, and Related Compounds, as Inhibitors of ER Stress-induced XBP1 Activation : II. Structure Elucidation
- SAR Study of a Novel Triene-ansamycin Group Compound, Quinotrierixin, and Related Compounds, as Inhibitors of ER Stress-induced XBP1 Activation : I. Taxonomy, Fermentation, Isolation, Biological Activities and SAR Study
- Trierixin, a Novel Inhibitor of ER Stress-induced XBP1 Activation from Streptomyces sp. : II. Structure Elucidation
- Antioxidative activity of the flower of Torenia fournieri
- Comparison of Two Vectors for Functional Expression of a Bacterial Cytochrome P450 Gene in Escherichia coli Using CYP153 Genes
- Directed Evolution of the Actinomycete Cytochrome P450 MoxA (CYP105) for Enhanced Activity