NAD(P)H Dehydrogenase-Dependent Cyclic Electron Flow around Photosystem I in the Cyanobacterium Synechocystis PCC 6803:a Study of Dark-Starved Cells and Spheroplasts : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS

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概要

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• Electron donation to P700^+ through plastoquinone in the intersystem chain from both respiratory substrates and the photoreductants in PSI has been shown to be mediated by the NAD(P)H-dehydrogenase complex (NDH) in Synechocystis PCC 6803 cells [Mi et al. (1992) Plant Cell Physiol. 33 : 1233]. To confirm the participation of NDH in the cyclic electron flow around PSI, the redox kinetics of P700 and Chl fluorescence were analyzed in cells rendered deficient in respiratory substrates by dark starvation and in spheroplasts. Dark-starved cells showed a high steady-state level of P700^+ under far-red (FR) illumination and the plastoquinone pool was in a highly oxidized state. An NDH-defective mutant consistently showed a high level of P700 oxidation under FR before and after the dark starvation. Donation of electrons either from exogenous NADPH or from photoreduced NADP^+ to the intersystem chain via plastoquinone was demonstrated using spheroplasts from wild-type cells, but not those from the NDH-defective mutant, as monitored by following changes in the kinetics of Chl fluorescence and the redox state of P700. The electron flow to PSI via plastoquinone, mediated by NADPH, was sensitive to rotenone, Hg^<2+> ions and 2-thenoyltrifluoroacetone, inhibitors of mitochondrial NDH and succinate dehydrogenase, but not to antimycin A. The pool size of electrons that can be donated to P700^+ from the cytosol through the intersystem chain increased with in-creasing duration of illumination time by actinic light and was sensitive to rotenone in both wild-type cells and spheroplasts, but no such results were obtained in the NDH-defective mutant of Synechocystis 6803 . The results support our previous conclusion that NDH is a mediator of both respiratory electron flow and cyclic electron flow around PSI to the intersystem chain in the cyanobacterium Synechocystis.

• 日本植物生理学会の論文

著者

• Asada Kozi

  Dept. Biotech Fac. Engin. Fukuyama Univ.

• Asada K

  The Research Institute For Food Science Kyoto University

• ENDO Tsuyoshi

  Kyoto Univ.Food Sci.

• SCHREIBER Ulrich

  Wurzburg Univ.

• Endo Tsuyoshi

  The Research Institute for Food Science, Kyoto University

• Asada Kozi

  The Research Institute for Food Science, Kyoto University

• Mi Hualing

  The Research Institute for Food Science, Kyoto University

• Schreiber Ulrich

  Julius-von-Sachs Institut fur Biowissenschaften der Universitat Wtirzburg

• Ogawa Teruo

  Solar Energy Research Group, The Institute of Physical and Chemical Research(Riken)

• Ogawa Teruo

  Bioscience Center Nagoya Univ.

• Ogawa T

  Nagoya Univ. Nagoya Jpn

• Asada Kozi

  The Research Institute For Food Science Kyoto University:(present)department Of Biotechnology Facult

• Endo Tsuyoshi

  Department Of Bioresource Science Obihiro University Of Agriculture And Veterinary Medicine

• Endo Tsuyoshi

  The Research Institute For Food Science Kyoto University

• Ogawa Teruo

  Solar Energy Research Group The Institute Of Physical And Chemical Research (riken)

• Schreiber U

  Univ. Wuerzburg Wuerzburg Deu

• Schreiber Ulrich

  Julius-von-sachs-institut Fur Biowissenschaften Mit Botanischen Garten Der Universitat Wurzburg

• Endo Tsuyoshi

  Department Of Plant Genes And Totipotency Graduate School Of Biostudies Kyoto University

• Endo Tsuyoshi

  Division Of Applied Life Sciences Graduae School Of Agriculture

• Asada Kozi

  The Research Institute For Food Science Kyoto Niversity

• Mi Hualing

  Shanghai Inst. Plant Physiol. The Chinese Academy Of Sciences

• Endo T

  Graduate School Of Biostudies Kyoto University

• Asada Kozi

  The Research Institulefor Fbod Science Kyoto University

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