Attachment of CuZn-Superoxide Dismutase to Thylakoid Membranes at the Site of Superoxide Generation (PSI) in Spinach Chloroplasts : Detection by Immuno-Gold Labeling After Rapid Freezing and Substitution Method : ENVIRONMENTAL AND STRESS RESPONSES : STRUC
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概要
- 論文の詳細を見る
In chloroplasts O^-_2 is photoproduced via the univalent reduction of O_2 in PSI even under conditions that are favorable for photosynthesis. The photogenerated O^-_2 is disproportionated to H_2O_2 and O_2 in a reaction that is catalyzed by superoxide dismutase (SOD). The H_2O_2-scavenging ascorbate peroxidase is bound to the thylakoid membranes at or near the PSI reaction center [Miyake and Asada (1992) Plant Cell Physiol. 33: 541], and the primary product of oxidation in the peroxidase-catalyzed reaction, the monodehydroascorbate radical, is photoreduced to ascorbate in PSI in a reaction mediated by ferredoxin [Miyake and Asada (1994) Plant Cell Physiol. 35: 539]. Therefore, SOD should be localized at or near the PSI complex. We report here the microcompartmentalization of the chloroplastic CuZn-SOD on the stromal-faces of thylakoid membranes where the PSI-complex is located. Spinach leaves were fixed and substituted by a rapid freezing and substitution method that allows visualization of intact chloroplasts. The embedded sections were immuno labeled with the antibody against CuZn-SOD by the immuno-gold method. About 70% of the immunogold particles were found within 5 nm from the surface of the stromal-faces of thylakoid membranes. Of these particles, about 40% were found at the ends and margins of the grana thylakoids and 60% were found on the stromal side of the stromal thylakoids. From these results, the local concentration of CuZn-SOD on the stroma-facing surfaces of the thylakoid membranes was estimated to be about 1 mM. The effect of the microcompartmentalization of CuZn-SOD on the scavenging of superoxide radicals is discussed.
- 日本植物生理学会の論文
著者
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Asada Kozi
Dept. Biotech Fac. Engin. Fukuyama Univ.
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Asada K
The Research Institute For Food Science Kyoto University
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Asada Kozi
The Research Institute for Food Science, Kyoto University
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Kanematsu Sumio
Department of Food Science and Technology, Minami-Kyushu University
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Kanematsu Sumio
Dept.food Sci.technol. Minami-kyushu Univ.
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Kanematsu S
Minami‐kyushu Univ. Miyazaki Jpn
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Kanematsu Sumio
Department Of Food Science And Technology Minami-kyushu University
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Ogawa Kenichi
Department of Botany, Faculty of Science, Kyoto University
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TAKABE Keiji
Department of Wood Science and Technology, Faculty of Agriculture, Kyoto University
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Asada Kozi
The Research Institute For Food Science Kyoto University:(present)department Of Biotechnology Facult
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Ogawa Kenichi
Seiko Instruments Inc.
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Ogawa Ken'ichi
Dep.bot. Fac.sci. Kyoto Univ.
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Asada Kozi
The Research Institute For Food Science Kyoto Niversity
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Takabe Keiji
Department Of Wood Science And Technology Faculty Of Agriculture Kyoto University
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Takabe Keiji
Department Of Wood Science & Technology Faculty Of Agriculture
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Ogawa K
Dep.bot. Fac.sci. Kyoto Univ.
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Ogawa Kenichi
Dep.bot. Fac.sci. Kyoto Univ.
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Asada Kozi
The Research Institulefor Fbod Science Kyoto University
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Ogawa Ken-ichi
Dep.bot. Fac.sci. Kyoto Univ.
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