Photoactivation of the Electron Flow from NADPH to Plastoquinone in Spinach Chloroplasts : GROWTH AND DEVEROPMENT : ENVIRONMENTAL AND STRESS RESPONSES
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概要
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Intact chloroplasts from spinach showed a transient increase in Chl fluorescence after saturating illumination with actinic light and its yield depended on the duration of illumination and the intensity of the actinic light (AL). The increase was partially suppressed when antimycin A was added immediately after termination of the AL. The inhibited fluorescence increase, therefore, reflected the electron flow from the reductant(s) that had accumulated during the actinic illumination to the plastoquinone (PQ) pool via ferredoxin and the antimycin A-sensitive Cyt b-559 [Miyake et al. (1995) Plant Cell Physiol. 36: 743]. Addition of dihydroxyacetone phosphate (DHAP) to chloroplasts caused the enhancement of the increase in fluorescence after AL, which was inhibited by antimycin A. Decay of the transiently raised fluorescence was retarded by 2-heptyl-4-hydroxyquinoline N-oxide and stigmatellin, suggesting that re-oxidation of the reduced PQ pool is coupled with the operation of Q-cycle. Although the activity of the stromal enzyme system that supplies NADPH on addition of DHAP was constant irrespective of light or darkness, the capacity of the intact chloroplasts to show a DHAP-dependent fluorescence increase had a limited lifetime after AL was turned off. This result suggests that the antimycin A-sensitive Cyt b-559 or ferredoxin-NADP reductase is activated by light and deactivated in the dark. In ruptured chloroplasts, the addition of NADPH increased the dark fluorescence yield only in the presence of Fd, which also was inhibited by antimycin A. Thus the photoregulatory mechanism of Cyt b-559 (Fd) in intact chloroplasts appeared to be lost when chloroplasts were ruptured.
- 日本植物生理学会の論文
著者
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Asada Kozi
Dept. Biotech Fac. Engin. Fukuyama Univ.
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Asada Kozi
Faculty Of Bioscience And Biotechnology Fukuyama University
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Asada K
The Research Institute For Food Science Kyoto University
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SCHREIBER Ulrich
Wurzburg Univ.
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Asada Kozi
The Research Institute for Food Science, Kyoto University
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Schreiber Ulrich
Julius-von-Sachs Institut fur Biowissenschaften der Universitat Wtirzburg
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Mano Junichi
The Research Institute for Food Science, Kyoto University
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Miyake Chikahiro
The Research Institute for Food Science, Kyoto University
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Asada Kozi
The Research Institute For Food Science Kyoto University:(present)department Of Biotechnology Facult
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Mano Jun'ichi
Faculty Of Agriculture Yamaguchi University
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Mano Junichi
The Research Institute For Food Science Kyoto University
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Schreiber U
Univ. Wuerzburg Wuerzburg Deu
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Schreiber Ulrich
Julius-von-sachs-institut Fur Biowissenschaften Mit Botanischen Garten Der Universitat Wurzburg
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Mano Junichi
Res. Inst. Food Sci. Kyoto University Uji Kyoto
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Miyake Chikahiro
Plant Physiology Research Group Research Institute Of Innovative Technology For The Earth (rite)
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Miyake Chikahiro
The Research Institute For Food Science Kyoto University
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Asada Kozi
The Research Institute For Food Science Kyoto Niversity
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Mano J
Faculty Of Agriculture Yamaguchi University
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Miyake C
Research Institute Of Innovative Technology For The Earth (rite)
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Asada Kozi
The Research Institulefor Fbod Science Kyoto University
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