Characteristic Amino Acid Sequences of Chloroplast and Cytosol Isozymes of CuZn-Superoxide Dismutase in Spinach, Rice and Horsetail
スポンサーリンク
概要
- 論文の詳細を見る
Two isozymes of CuZn-superoxide dismutase (SOD) were purified from spinach. One (CuZn-SOD II) was localized in chloroplasts and had the same properties as the enzyme previously reported [Asada et al. (1973) Eur. J. Biochem. 36: 257-266]. The other isozyme (CuZn-SOD I) was predominantly expressed in seeds and in etiolated seedlings of spinach, but was localized in the cytosol of the leaves as a minor enzyme. The isozymes have similar molecular weights, subunit structures, and metal contents; but their amino acid compositions, absorption spectra, CD spectra and sensitivities to hydrogen peroxide are different. The amino acid sequences of 50 amino-terminal residues of the chloroplast and cytosol isozymes of CuZn-SOD from spinach, rice and horsetail were determined and compared with those of CuZn-SODs from other plants. The sequences can be divided into chloroplast and cyto-sol types, and characteristic sequences can be identified in accordance with the observations that the two types of CuZn-SOD isozymes from green algae, ferns and angiosperms can be distinguished immunologically from each other. Differences in amino acid sequences among the cytosol enzymes are greater than those among the chloroplast enzymes, indicating that the rate of mutation of the cytosol CuZn-SOD is higher than that of the chloroplast CuZn-SOD. These results provide further evidence that the divergence of the two types of isozyme of CuZn-SOD occurred at a very early stage of its acquisition, and that each type of CuZn-SOD has evolved independently.
- 日本植物生理学会の論文
著者
-
Asada Kozi
The Research Institute for Food Science, Kyoto University
-
Kanematsu Sumio
Department Of Food Science And Technology Minami-kyushu University
-
Kanematsu Sumio
The Research Institute For Food Science Kyoto University
-
Asada Kozi
The Research Institute For Food Science Kyoto Niversity
-
Asada Kozi
The Research Institulefor Fbod Science Kyoto University
関連論文
- Suppression of Quantum Yield of Photosystem II by Hyperosmotic Stress in Chlamydomonas reinhardtii : ENVIRONMENTAL AND STRESS RESPONSES
- Quenching Analysis of Chlorophyll Fluorescence by the Saturation Pulse Method : Particular Aspects Relating to the Study of Eukaryotic Algae and Cyanobacteria : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Thylakoid Membrane-Bound, NADPH-Specific Pyridine Nucleotide Dehydrogenase Complex Mediates Cyclic Electron Transport in the Cyanobacterium Synechocystis sp. PCC 6803 : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
- NAD(P)H Dehydrogenase-Dependent Cyclic Electron Flow around Photosystem I in the Cyanobacterium Synechocystis PCC 6803:a Study of Dark-Starved Cells and Spheroplasts : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Electron Donation from Cyclic and Respiratory Flows to the Photosynthetic Intersystem Chain is Mediated by Pyridine Nucleotide Dehydrogenase in the Cyanobacterium Synechocystis PCC 6803 : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Donation of Electrons from Cytosolic Components to the Intersystem Chain in the Cyanobacterium Synechococcus sp. PCC 7002 as Determined by the Reduction of P700^+ : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Generation of Superoxide Anion and Localization of CuZn-Superoxide Dismutase in the Vascular Tissue of Spinach Hypocotyls : Their Association with Lignification
- MOLECULAR PROPERTIES OF CuZn-SUPEROXIDE DISMUTASE ISOZYMES FROM EUKARYOTIC ALGA, Spirogyra sp.
- TOBACCO CHLOROPLASTIC CuZn-SUPEROXIDE DISMUTASE CANNOT FUNCTION WITHOUT ITS LOCALIZATION AT THE SITE OF SUPEROXIDE GENERATION (PS I)
- GENERATION OF SUPEROXIDE AND LIGNIN SYNTHESIS IN SPINACH HYPOCOTYL
- INTERACTION OF SPINACH CHLOROPLASTIC CuZn-SUPEROXID DISMUTASE WITH THE THYLAKOID MEMBRANES
- INTRA-AND EXTRACELLULAR LOCALIZATION OFCYTOSOLIC CuZn-SUPEROXIDE DISMUTASE IN SPINACH LEAVES
- Intra- and Extra-Cellular Localization of "Cytosolic" CuZn-Superoxide Dismutase in Spinach Leaf and Hypocotyl
- Attachment of CuZn-Superoxide Dismutase to Thylakoid Membranes at the Site of Superoxide Generation (PSI) in Spinach Chloroplasts : Detection by Immuno-Gold Labeling After Rapid Freezing and Substitution Method : ENVIRONMENTAL AND STRESS RESPONSES : STRUC
- Electron Flow to the Intersystem Chain from Stromal Components and Cyclic Electron Flow in Maize Chloroplasts, as Detedted in Intact Leaves by Monitoring Redox Change of P700 and Chlorophyll Fluorescence
- Pool Size of Electrons That Can Be Donated to P700^+, As Determined in Intact Leaves : Donation to P700^+ from Stromal Components Via the Intersystem Chain : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Increased Levels of Monodehydroascorbate Radical in UV-B-Irradiated Broad Bean Leaves
- Monodehydroascorbate Radical Detected by Electron Paramagnetic Resonance Spectrometry Is a Sensitive Probe of Oxidative Stress in Intact Leaves
- Photoactivation of the Electron Flow from NADPH to Plastoquinone in Spinach Chloroplasts : GROWTH AND DEVEROPMENT : ENVIRONMENTAL AND STRESS RESPONSES
- Ferredoxin-Dependent and Antimycin A-Sensitive Reduction of Cyiochrome b-559 by Far-Red Light in Maize Thylakoids : Participation of a Menadiol-Reducible Cytochrome b-559 in Cyclic Electron Flow : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENER
- CuZn-Superoxide Dismutases from the Fern Equisetum arvense and the Green Alga Spirogyra sp.: Occurrence of Chloroplast and Cytosol Types of Enzyme
- Dark Induction of the Non-Photochemical Quenching of Chlorophyll Fluorescence by Acetate in Chlamydomonas reinhardtii
- Effect of sulfate ions on photophosphorylation by spinach chloroplasts
- Induction of Enzymes Involved in the Ascorbate-Dependent Antioxidative System, Namely, Ascorbate Peroxidase, Monodehydroascorbate Reductase and Dehydroascorbate Reductase, after Exposure to Air of Rice (Oryza sativa) Seedlings Germinated under Water
- The Amino Acid Sequence of Ascorbate Peroxidase from Tea Has a High Degree of Homology to That of Cytochrome c Peroxidase from Yeast
- The growth promoting effect of phytic acid on callus tissues of rice seed
- Phosphorylation of myo-inositol in ripening grains of rice and wheat. Incorporation of phosphate-^P and myo-inositol-^3H into myo-inositol phosphates
- Reduction of plastocyanin with O_2^- and superoxide dismutase-dependent oxidation of plastocyanin by H_2O_2
- Superoxide dismutases from kidney bean leaves
- Characteristic Amino Acid Sequences of Chloroplast and Cytosol Isozymes of CuZn-Superoxide Dismutase in Spinach, Rice and Horsetail
- Occurrence of Cu,Zn-Superoxide Dismutase in the Intrathylakoid Space of Spinach Chloroplasts
- Monodehydroascorbate Reductase in Spinach Chloroplasts and Its Participation in Regeneration of Ascorbate for Scavenging Hydrogen Peroxide
- The Processing of a 57-kDa Precursor Peptide to Subunits of Rice Glutelin
- Purification of Dehydroascorbate Reductase from Spinach and Its Characterization as a Thiol Enzyme
- Scavenging of Hydrogen Peroxide in Prokaryotic and Eukaryotic Algae: Acquisition of Ascorbate Peroxidase during the Evolution of Cyanobacteria
- Inactivation of Ascorbate Peroxidase by Thiols Requires Hydrogen Peroxide
- cDNA Cloning of Monodehydroascorbate Radical Reductase from Cucumber : a High Degree of Homology in Terms of Amino Acid Sequence between This Enzyme and Bacterial Fiavoenzymes : ENVIRONMENTAL AND STRESS RESPONSES : GENES STRUCTURE AND EXPRESSION
- Separate Assays Specific for Ascorbate Peroxidase and Guaiacol Peroxidase and for the Chloroplastic and Cytosolic Isozymes of Ascorbate Peroxidase in Plants : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- Inactivation of Ascorbate Peroxidase in Spinach Chloroplasts on Dark Addition of Hydrogen Peroxide : Its Protection by Ascorbate
- Dark Anaerobic Inactivation of Photosynthetic Oxygen Evolution by Chlamydomonas reinhardtii
- Paraquat Resistant Tobacco Calluses with Enhanced Superoxide Dismutase Activity
- Purification of Ascorbate Peroxidase in Spinach Chloroplasts : Its Inactivation in Ascorbate-Depleted Medium and Reactivation by Monodehydroascorbate Radical
- Ferredoxin-Dependent Photoreduction of the Monodehydroascorbate Radical in Spinach Thylakoids : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- CuZn-Superoxide Dismutases in Rice: Occurrence of an Active, Monomeric Enzyme and Two Types of Isozyme in Leaf and Non-Photosynthetic Tissues
- Inactivation Mechanism of Ascorbate Peroxidase at Low Concentrations of Ascorbate; Hydrogen Peroxide Decomposes Compound I of Ascorbate Peroxidase
- Purification and Molecular Properties of the Thylakoid-Bound Ascorbate Peroxidase in Spinach Chloroplasts
- Molecular Sizes of the Catalytic Units of Oxygen Evolution and the Reaction Center in Photosystem II of Spinach Thylakoids
- Thylakoid-Bound Ascorbate Peroxidase in Spinach Chloroplasts and Photoreduction of Its Primary Oxidation Product Monodehydroascorbate Radicals in Thylakoids : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
- Ascorbate Peroxidase in Tea Leaves: Occurrence of Two Isozymes and the Differences in Their Enzymatic and Molecular Properties
- Selective lodo-Labeling of an Intrinsic Electron Donor of Photosystem II in Illuminated Tris-Treated Thylakoids
- Purification of cytochrome f from Japanese-radish leaves
- Manganese binding to sodium cyanide-treated chloroplasts : Effects of light and redox-potentials on the binding
- Oxidation Rate of Monomeric Radish Cytochrome f with Plastocyanin
- Chloroplast Development in 4-Thiouridine-Cultued Radish Seedlings V. Protein Synthesis in Vivo
- Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
- Purification and properties of cytochrome c and two peroxidases from spinach leaves
- Conservation of electron transport and energy transfer reactions of spinach chloroplasts in glycerol
- Photoautotrophy and the photosynthetic potential of chlorophyllous cells in mixotrophic cultures
- A Manganese Protein from Thylakoids of a Cyanobacterium, Plectonema boryanum : Inhibition of Oxygen Evolution with Its Antibody
- Spinach chloroplasts scavenge hydrogen peroxide on illumination
- Dependence of Oxygen Affinity for Mehler Reaction on Photochemical Activity of Chloroplast Thylakoids