Purification and cDNA Cloning of Chloroplastic Monodehydroascorbate Reductase from Spinach

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概要

• 論文の詳細を見る

• The chloroplastic isoform of monodehydroascorbate (MDA) radical reductase was purified from spinach chloroplasts and leaves. The cDNA of chloroplastic MDA reductase was cloned, and its deduced amino acid sequence, consisting of 497 residues, showed high homology with those of putative organellar MDA reductases deduced from cDNAs of several plants. The amino acid sequence of the amino terminal of the purified enzyme suggested that the chloroplastic enzyme has a transit peptide consisting of 53 residues. A southern blot analysis suggested the occurrence of a gene encoding another isoform homologous to the chloroplastic isoform in spinach. The recombinant enzyme was highly expressed in Eschericia coli using the cDNA, and purified to a homogeneous state with high specific activity. The enzyme properties of the chloroplastic isoform are presented in comparison with those of the cytosolic form.

• 社団法人 日本農芸化学会の論文
• 2005-04-23

著者

• HOSSAIN M.

  Department of Applied Quantum Physics and Nuclear Engineering, Kyushu University

• Matsuo Michinori

  Lab. Of Cellular Biochemistry Div. Of Applied Life Sciences Graduate School Of Agriculture Kyoto Uni

• Asada K

  Fukuyama Univ. Hiroshima Jpn

• Asada Kozi

  Dept. Biotech Fac. Engin. Fukuyama Univ.

• Asada Kozi

  Faculty Of Bioscience And Biotechnology Fukuyama University

• Asada K

  The Research Institute For Food Science Kyoto University

• SANO Satoshi

  Graduate School of Agriculture, Kyoto Prefectural University

• TAO Satoru

  Graduate School of Agriculture, Kyoto Prefectural University

• ENDO Yuko

  Graduate School of Agriculture, Kyoto Prefectural University

• INABA Tomomi

  Graduate School of Agriculture, Kyoto Prefectural University

• MIYAKE Chikahiro

  Plant Physiology Research Group, Research Institute of Innovative Technology for the Earth (RITE)

• MATSUO Michinori

  Graduate School of Agriculture, Kyoto University

• AOKI Hideyuki

  Hokuriku Research Center, National Agricultural Research Center (NARC)

• SAITO Kazumi

  Graduate School of Agriculture, Kyoto Prefectural University

• SANO Satoshi

  Fac. Agr., Kyoto Pref. Univ.

• HOSSAIN Mohamed

  Dept. Biochem., Dhaka Univ.

• Asada Kozi

  The Research Institute For Food Science Kyoto University:(present)department Of Biotechnology Facult

• Tao Satoru

  Graduate School Of Agriculture Kyoto Prefectural University

• Saito Kazumi

  Graduate School Of Agriculture Kyoto Prefectural University

• Sano Satoshi

  Graduate School Of Agriculture Kyoto Prefectural University

• Hossain Mohamed

  Department Of Biochemistry University Of Dhaka

• Aoki Hideyuki

  Hokuriku Research Center National Agricultural Research Center (narc)

• Miyake Chikahiro

  Plant Physiology Research Group Research Institute Of Innovative Technology For The Earth (rite)

• Miyake Chikahiro

  Res. Inst. Food Sci. Kyoto Univ.

• Hossain M.

  Department Of Applied Quantum Physics And Nuclear Engineering Kyushu University

• Matsuo Michinori

  Graduate School Of Agriculture Kyoto University

• Miyake C

  Research Institute Of Innovative Technology For The Earth (rite)

• Miyake Chikahiro

  Kobe Univ. Kobe Jpn

• Endo Yuko

  Graduate School Of Agriculture Kyoto Prefectural University

• Inaba Tomomi

  Graduate School Of Agriculture Kyoto Prefectural University

• Aoki Hideyuki

  Hokuriku Research Center Narc

• Asada Kozu

  The Research Institute For Food Science Kyoto University

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