The FAD-Enzyme Monodehydroascorbate Radical Reductase Mediates Photoproduction of Superoxide Radicals in Spinach Thylakoid Membranes
スポンサーリンク
概要
- 論文の詳細を見る
The photoreduction of dioxygen in spinach thylakoid membranes was enhanced about 10-fold by the FAD-enzyme monodehydroascorbate radical (MDA) reductase at 1 μM. The primary photoreduced product of dioxygen catalyzed by MDA reductase was the superoxide radical, as evidenced by the inhibition of photoreduction of Cyt c by superoxide dismutase. The apparent K_m for dioxygen of the MDA reductase-dependent photoreduction of dioxygen was 100 μM, higher by one order of magnitude than that observed with thylakoid membranes only. Glutathione reductase, ferredoxin-NADP^+ reductase, and glycolate oxidase also mediated the photoproduction of superoxide radicals in thylakoid membranes at rates similar to those with MDA reductase. Among these flavoenzymes, MDA reductase is the most likely mediator stimulating the photoreduction of dioxygen in chloroplasts;its function in the protection from photoinhibition under excess light is discussed.
- 日本植物生理学会の論文
著者
-
Asada Kozi
Department Of Biotechnology Faculty Of Life Science And Biotechnology Fukuyama University
-
Asada K
Fukuyama Univ. Hiroshima Jpn
-
Asada Kozi
Dept. Biotech Fac. Engin. Fukuyama Univ.
-
Asada Kozi
Faculty Of Bioscience And Biotechnology Fukuyama University
-
Asada K
The Research Institute For Food Science Kyoto University
-
SCHREIBER Ulrich
Wurzburg Univ.
-
Schreiber Ulrich
Julius-von-Sachs Institut fur Biowissenschaften der Universitat Wtirzburg
-
SANO Satoshi
Fac. Agr., Kyoto Pref. Univ.
-
Miyake Chikahiro
Graduate School of Biological Science, Nara Institute of Science and Technology
-
Hormann Henning
Julius-von-Sachs-Institut fur Biowissenschaften mit Botanischen Garten der Universitat Wurzburg
-
Sano Satoshi
Research Institute of Innovative Technology for the Earth (RITE)
-
Asada Kozi
The Research Institute For Food Science Kyoto University:(present)department Of Biotechnology Facult
-
Asada Kozi
Department Of Biotechnology Faculty Of Engineering Fukuyama University
-
Sano Satoshi
Graduate School Of Agriculture Kyoto Prefectural University
-
Sano Satoshi
Research Institute For Innovative Technology For The Earth (rite)
-
Schreiber U
Univ. Wuerzburg Wuerzburg Deu
-
Schreiber Ulrich
Julius-von-sachs-institut Fur Biowissenschaften Mit Botanischen Garten Der Universitat Wurzburg
-
Miyake Chikahiro
Plant Physiology Research Group Research Institute Of Innovative Technology For The Earth (rite)
-
Miyake Chikahiro
Res. Inst. Food Sci. Kyoto Univ.
-
Miyake C
Research Institute Of Innovative Technology For The Earth (rite)
-
Miyake Chikahiro
Kobe Univ. Kobe Jpn
-
Asada Kozu
The Research Institute For Food Science Kyoto University
関連論文
- Properties of the Respiratory NAD(P)H Dehydrogenase Isolated from the Cyanobacterium Synechocystis PCC6803
- MOLECULAR SPECIES OF NADPH-SPECIFIC NDH IN STROMA OF HIGHER PLANTS
- NAD(P)H Dehydrogenase-Dependent, Antimycin A-Sensitive Electron Donation to Plastoquinone in Tobacco Chloroplasts
- Isolation of a Novel NAD(P)H-Quinone Oxidoreductase from the Cyanobacterium Synechocystis PCC6803
- Donation of Electrons to Plastoquinone by NAD(P)H Dehydrogenase and by Ferredoxin-Quinone Reductase in Spinach Chloroplasts
- INHIBITION OF NAD(P)H DEGYDROGENASE (NDH) BY HQNO IN THE CYANOBACTERIUM Synechocystis PCC 6803
- PURIFICATION OF NAD(P)H DEHYDROGENASE COMPLEX (NDH) IN Synechocystis PCC 6803
- CYCLIC ELECTRON TRANSPORT MEDIATED BY NAD(P)H DEHYDROGENASE IN TOBACCO CHLOROPLASTS
- NAD(P)H DEHYDROGENASE-DEPENDENT CYCLIC ELECTRON FLOW AROUND PHOTOSYSTEM I IN CYANOBACTERIA
- INDUCTION AND PURIFICATION OF NAD(P)H DEHYDROGENASE COMPLEX(NDH)IN Synechocystis PCC 6803
- Suppression of Quantum Yield of Photosystem II by Hyperosmotic Stress in Chlamydomonas reinhardtii : ENVIRONMENTAL AND STRESS RESPONSES
- Quenching Analysis of Chlorophyll Fluorescence by the Saturation Pulse Method : Particular Aspects Relating to the Study of Eukaryotic Algae and Cyanobacteria : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Thylakoid Membrane-Bound, NADPH-Specific Pyridine Nucleotide Dehydrogenase Complex Mediates Cyclic Electron Transport in the Cyanobacterium Synechocystis sp. PCC 6803 : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
- NAD(P)H Dehydrogenase-Dependent Cyclic Electron Flow around Photosystem I in the Cyanobacterium Synechocystis PCC 6803:a Study of Dark-Starved Cells and Spheroplasts : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Electron Donation from Cyclic and Respiratory Flows to the Photosynthetic Intersystem Chain is Mediated by Pyridine Nucleotide Dehydrogenase in the Cyanobacterium Synechocystis PCC 6803 : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Donation of Electrons from Cytosolic Components to the Intersystem Chain in the Cyanobacterium Synechococcus sp. PCC 7002 as Determined by the Reduction of P700^+ : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Purification and cDNA Cloning of Chloroplastic Monodehydroascorbate Reductase from Spinach
- PURIFICATION AND cDNA CLONING OF CHLOROPLASTIC MONODEHYDROASCORBATE REDUCTASE FROM SPINACH
- Generation of Superoxide Anion and Localization of CuZn-Superoxide Dismutase in the Vascular Tissue of Spinach Hypocotyls : Their Association with Lignification
- GENERATION OF SUPEROXIDE IS ACCOMPANIED WITH BIOSYNTHESIS OF LIGNIN IN TRACHEARY ELEMENT DIFFERENTIATION OF CULTURED ZINNIA ELEGANS CELLS
- MOLECULAR PROPERTIES OF CuZn-SUPEROXIDE DISMUTASE ISOZYMES FROM EUKARYOTIC ALGA, Spirogyra sp.
- TOBACCO CHLOROPLASTIC CuZn-SUPEROXIDE DISMUTASE CANNOT FUNCTION WITHOUT ITS LOCALIZATION AT THE SITE OF SUPEROXIDE GENERATION (PS I)
- GENERATION OF SUPEROXIDE AND LIGNIN SYNTHESIS IN SPINACH HYPOCOTYL
- INTERACTION OF SPINACH CHLOROPLASTIC CuZn-SUPEROXID DISMUTASE WITH THE THYLAKOID MEMBRANES
- INTRA-AND EXTRACELLULAR LOCALIZATION OFCYTOSOLIC CuZn-SUPEROXIDE DISMUTASE IN SPINACH LEAVES
- Intra- and Extra-Cellular Localization of "Cytosolic" CuZn-Superoxide Dismutase in Spinach Leaf and Hypocotyl
- Attachment of CuZn-Superoxide Dismutase to Thylakoid Membranes at the Site of Superoxide Generation (PSI) in Spinach Chloroplasts : Detection by Immuno-Gold Labeling After Rapid Freezing and Substitution Method : ENVIRONMENTAL AND STRESS RESPONSES : STRUC
- GENE DISRUPTION OF NDHB IMPAIRS CYCLIC ELECTRON FLOW AROUND PS I IN TOBACCO
- Electron Flow to the Intersystem Chain from Stromal Components and Cyclic Electron Flow in Maize Chloroplasts, as Detedted in Intact Leaves by Monitoring Redox Change of P700 and Chlorophyll Fluorescence
- Pool Size of Electrons That Can Be Donated to P700^+, As Determined in Intact Leaves : Donation to P700^+ from Stromal Components Via the Intersystem Chain : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENERGETICS
- Methyl Viologen-Dependent Cyclic Electron Transport in Spinach Chloroplasts in the Absence of Oxygen
- The FAD-Enzyme Monodehydroascorbate Radical Reductase Mediates Photoproduction of Superoxide Radicals in Spinach Thylakoid Membranes
- Increased Levels of Monodehydroascorbate Radical in UV-B-Irradiated Broad Bean Leaves
- ESTIMATION OF ASCORBATE PEROXIDASE ACTIVITY IN CHLOROPLASTS BY MONITORING MONODEHYDROASCORBIC ACID
- Monodehydroascorbate Radical Detected by Electron Paramagnetic Resonance Spectrometry Is a Sensitive Probe of Oxidative Stress in Intact Leaves
- Monodehydroascorbate radical reductase-dependent photoreduction of oxygen in chloroplast thylakoids
- ACTIVE OXYGEN AND MONODEHYDROASCORBATE RADICAL PRODUCTION IN VICIA FABA LEAVES AND ISOLATED THYLAKOID MEMBRANES UPON UV-B IRRADIATION
- DETECTION OF PHOTOOXIDATIVE STRESS BY MONODEHYDROASCORBATE IN LEAVES
- Photoactivation of the Electron Flow from NADPH to Plastoquinone in Spinach Chloroplasts : GROWTH AND DEVEROPMENT : ENVIRONMENTAL AND STRESS RESPONSES
- Ferredoxin-Dependent and Antimycin A-Sensitive Reduction of Cyiochrome b-559 by Far-Red Light in Maize Thylakoids : Participation of a Menadiol-Reducible Cytochrome b-559 in Cyclic Electron Flow : ENVIRONMENTAL AND STRESS RESPONSES : MEMBRANES AND BIOENER
- Ferredoxin-dependent and antimycinA-sensitive reduction of cytochrome b559 in maize thylakoids:Menadiol-reducible, low-potential cytochrome b559 isferredoxin-quinone reductase
- Cooperation of Photosynthetic Reaction Center of Photosystem II under Weak Light as Shown by Light Intensity-dependence of the Half-effective Dose of Atrazine
- Isolation of the D1 Protein and a 28-kDa Fragment of the D2 Protein from Spinach Photosystem II Membranes
- RESISTANCE TO ENVIRONMENTAL STRESS IN TRANSGENIC TOBACCO OVEREXPRESSING OF CATALASE FROM E. coli IN CHLOROPLASTS
- Induction of Enzymes Involved in the Ascorbate-Dependent Antioxidative System, Namely, Ascorbate Peroxidase, Monodehydroascorbate Reductase and Dehydroascorbate Reductase, after Exposure to Air of Rice (Oryza sativa) Seedlings Germinated under Water
- DIFFERENTIAL RESPONSES OF ANTIOXICATIVEENZYMES IN RICE SEEDLINGS TO HYPOXIC STRESS
- DIVISION OF CULTURED ZINNIA ELEGANS CELLS IS REGULATED BY ACTIVE SPECIES OF OXYGEN
- Phenylethylamine-Induced Generation of Reactive Oxygen Species and Ascorbate Free Radicals in Tobacco Suspension Culture: Mechanism for Oxidative Burst Mediating Ca^ Influx
- Aromatic Monoamine-Induced Immediate Oxidative Burst Leading to an Increase in Cytosolic Ca^ Concentration in Tobacco Suspension Culture
- The NADPH : Quinone Oxidoreductase P1-ζ-crystallin in Arabidopsis Catalyzes the α, β-Hydrogenation of 2-Alkenals : Detoxication of the Lipid Peroxide-Derived Reactive Aldehdes
- ENZYMIC CHARACTERS AND OVEREXPRESSION IN TOBACCO OF Arabidopsis P1/Z-CRYSTALLIN
- PLANT ζ-CRYSTALLIN (Arabidopsis P1 PROTEIN) IS A NADPH-DIAMIDE REDUCTASE
- PHYTOPHENOXYL RADICAL AS A SUBSTRATE FOR MONODEHYDROASCORBATE REDUCTASE
- Characterization of Ascorbate Peroxidases from Unicellular Red Alga Galdieria partita
- Ferredoxin Limits Cyclic Electron Flow around PSI (CEF-PSI) in Higher Plants-Stimulation of CEF-PSI enhances Non-Photochemical Quenching of Chl Fluorescence in Transplastomic Tobacco
- Effects of Light Intensity on Cyclic Electron Flow Around PSI and its Relationship to Non-photochemical Quenching of Chl Fluorescence in Tobacco Leaves
- Stable Form of Ascorbate Peroxidase from the Red Alga Galdieria partita Similar to Both Chloroplastic and Cytosolic Isoforms of Higher Plants(Biochemistry & Molecular Biology)
- Formate Protects Photosynthetic Machinery from Photoinhibition
- Hydroxyl Radical Scavenging Activity of Citrulline : Implication for Drought-tolerance of Wild Watermelon
- ANALYSIS OF DROUGHT-INDUCED METALLOTHIONEIN GENE IN WILD-TYPE WATERMELON (Citrullus lanatus)
- Responses of Wild Watermelon to Drought Stress:Accumulation of an ArgE Homologue and Citrulline in Leaves during Water Deficits
- Photoreduction of ^O_2 and H_2^O_2 with Concomitant Evolution of ^O_2 in Intact Spinach Chloroplasts : Evidence for Scavenging of Hydrogen Peroxide by Peroxidase
- A strategy for Desert Afforestation Using Plastid Transformation Technique for CO_2 Sequestration
- Photoinactivation of Ascorbate Peroxidase in Isolated Tobacco Chloroplasts : Galdieria partita APX Maintains the Electron Flux through the Water-Water Cycle in Transplastomic Tobacco Plants
- CO_2 Response of Cyclic Electron Flow around PSI (CEF-PSI) in Tobacco Leaves-Relative Electron fluxes through PSI and PSII Determine the Magnitude of Non-photochemical Quenching (NPQ) of Chl Fluorescence
- Enhancement of Cyclic Electron Flow Around PSI at High Light and its Contribution to the Induction of Non-Photochemical Quenching of Chl Fluorescence in Intact Leaves of Tobacco Plants
- Purification and Characterization of Chloroplast Dehydroascorbate Reductase from Spinach Leaves
- PURIFICATION OF CHLOROPLAST DEHYDROASCORBATE REDUCTASE FROM SPINACH LEAVES AND ITS MOLECULAR CHARACTERIZATION
- Molecular analysis of drought-tolerant with watermelon from the Botawana desert
- THIOREDOXIN PEROXIDASE IN CYANOBACTERIA, Synechocystis sp. PCC6803 AND Synechococcus sp. PCC7942.
- Photoproduction of the Azidyl Radical from the Azide Anion on the Oxidizing Side of Photosystem II and Suppression of Photooxidation of Tyrosine Z by the Azidyl Radical : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
- Purification and Characterization of Class-I and Class-II Fructose-1,6-bisphosphate Aldolases from the Cyanobacterium Synechocytis sp. PCC6803
- CHLOROPLASTIC ASCORBATE PEROXIDASE IS THE PRIMARY TARGET OF METHYLVIOLOGENINDUCED PHOTOOXIDA TIVE STRESS IN LEAVES
- CLONING OF Azotobacter vinelandii Fe-SUPEROXIDE DISMUTASE GENE AND ITS REGULATED EXPRESSION IN E. coli CELLS
- Imaging of NPQ and ROS Formation in Tobacco Leaves : Heat Inactivation of the Water-Water Cycle Prevents Down-Regulation of PSII
- Detection of Singlet Oxygen and Superoxide with Fluorescent Sensors in Leaves Under Stress by Photoinhibition or UV Radiation
- Acclimation of Tobacco Leaves to High Light Intensity Drives the Plastoquinone Oxidation System-Relationship Among the Fraction of Open PSII Centers, Non-Photochemical Quenching of Chl Fluorescence and the Maximum Quantum Yield of PSII in the Dark
- Cyclic Electron Flow within PSII Protects PSII from its Photoinhibition in Thylakoid Membranes from Spinach Chloroplasts
- Determination of the Rate of Photoreduction of O_2 in the Water-Water Cycle in Watermelon Leaves and Enhancement of the Rate by Limitation of Photosynthesis
- Physiological Functions of the Water-Water Cycle (Mehler Reaction) and the Cyclic Electron Flow around PSI in Rice Leaves
- Cyclic Electron Flow within PSII Functions in Intact Chloroplasts from Spinach Leaves
- Cyt b-559 (Fd) Participating in Cyclic Electron Transport in Spinach Chloroplasts : Evidence for Kinetic Connection with the Cyt b_6/ƒ Complex
- Cyclic Flow of Electrons within PSII in Thylakoid Membranes
- MOLECULAR MECHANISM FOR FIXATION OF SOLAR ENERGY IN PLANTS WITHOUT SUNBURN : UV-SCREEN IN EPIDERMAL CELLS AND DISSIPATION OF EXCESS PHOTON ENERGY IN CHLOROPLASTS VIA THE WATER-WATER CYCLE
- Cyclic electron flow around PSI functions in the photoinhibited rice leaves(Plant Nutrition)
- Defense response of a pepper cultivar cv. Sy-2 is induced at temperatures below 24℃