Generation of Superoxide Anion and Localization of CuZn-Superoxide Dismutase in the Vascular Tissue of Spinach Hypocotyls : Their Association with Lignification

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概要

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• The sites of generations of superoxide anions and hydrogen peroxide in cross sections of hypocotyls from spinach seedlings were located by staining with nitroblue tetrazolium (NBT) and with starch-iodide, respectively. Formazan, produced upon the reduction of NBT by super-oxide, was observed mainly in the vascular tissue only in the presence of inhibitors of CuZn-superoxide dismutase (CuZn-SOD), and its formation was suppressed under an-aerobic conditions. Thus, NBT was reduced to formazan specifically by the superoxide anions generated in vascular tissue. The reduction of NBT was suppressed by inhibitors of NAD(P)H oxidase, but neither by cyanide nor azide, indicating the involvement of NAD(P)H oxidase in the generation of superoxide anions in the vascular tissue. Starch-I_2 complex also was formed in the vascular tissue, but not in the presence of either the CuZn-SOD inhibitor or the NAD(P)H oxidase inhibitor, indicating that the hydrogen peroxide is produced via the catalytic disproportionation with CuZn-SOD of the superoxide generated by NAD(P)H oxidase. Generations of superoxide anions and hydrogen peroxide in the vascular tissue were particularly apparent in the xylem and associated with the sites of distribution of CuZn-SOD as detemtined by an immunohistochemical method, and also with the location of lignin as determined by the phloroglucin-HCI reaction.

• 日本植物生理学会の論文

著者

• Asada K

  Fukuyama Univ. Hiroshima Jpn

• Asada Kozi

  Dept. Biotech Fac. Engin. Fukuyama Univ.

• Asada Kozi

  Faculty Of Bioscience And Biotechnology Fukuyama University

• Asada Kozi

  Res.inst.food Sci. Kyoto Univ.

• Asada K

  The Research Institute For Food Science Kyoto University

• Asada Kozi

  The Research Institute for Food Science, Kyoto University

• Kanematsu Sumio

  Department of Food Science and Technology, Minami-Kyushu University

• Kanematsu Sumio

  Dept.food Sci.technol. Minami-kyushu Univ.

• Kanematsu S

  Minami‐kyushu Univ. Miyazaki Jpn

• Kanematsu Sumio

  Department Of Food Science And Technology Minami-kyushu University

• Asada Kozi

  The Research Institute For Food Science Kyoto University:(present)department Of Biotechnology Facult

• Ogawa Kenichi

  Seiko Instruments Inc.

• Ogawa Ken'ichi

  Dep.bot. Fac.sci. Kyoto Univ.

• Asada K

  Dept. Biotech Fac. Engin. Fukuyama Univ.

• Asada Kozi

  The Research Institute For Food Science Kyoto Niversity

• Ogawa K

  Dep.bot. Fac.sci. Kyoto Univ.

• Ogawa Kenichi

  Dep.bot. Fac.sci. Kyoto Univ.

• Asada Kozu

  The Research Institute For Food Science Kyoto University

• Asada Kozi

  The Research Institulefor Fbod Science Kyoto University

• Ogawa Ken-ichi

  Dep.bot. Fac.sci. Kyoto Univ.

• Ogawa Ken′ichi

  Department of Botany, Faculty of Science, Kyoto University

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