Effects of the Degree of Polymerization on the Binding of Xyloglucans to Cellulose : STRUCTURE AND FUNCTION OF CELLS

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概要

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• Xyloglucan oligosaccharides were isolated with various degrees of polymerization (DP) and reduced with tritiated sodium borohydride. The 3^H-oligosaccharides were tested for their ability to bind to amorphous and microcrystalline celluloses and to cellulose filter paper. The time course of binding indicated that the radiolabeled oligosaccharides continued to be bound for at least 1 h after heating at 120℃. The binding probably required the organization of the oligosaccharides and celluloses by gradual annealing after heating. Although neither pentasaccharide (glucose : xylose, 3 : 2), heptasaccharide (glucose : xylose, 4 : 3) and nonasaccharide (glucose : xylose : galactose : fucose, 4 : 3 : 1 : 1) failed to bind to the celluloses, binding occurred with oligosaccharides with DP equivalent to more than four consecutive 1,4-β-glucosyl residues. The extent of binding to the celluloses increased gradually from octasaccharide (glucose : xylose, 5 : 3) to hendecosanosaccharide (glucose/xylose, 12 : 9), with the increase in the DP of 1,4-β-glucosyl residues. The binding of reduced cello-dextrins to cellulose required at least 4 consecutive I ,4-P-glucosyl residues. The extent of binding of cellopentitol or cellohexitol to cellulose was similar to that of hendecosanosaccharide, showing lower binding for xyloglucan oligosaccharides in spite of longer chains of 1,4-β-glucosyl residues. These findings suggest that the mode of binding to cellulose of xyloglucan oligosaccharides is different from that of cello-oligosaccharides.

• 日本植物生理学会の論文

著者

• Tada T

  Dep. Of Advanced Bioscience Fac. Of Agriculture Kinki Univ.

• Ogawa K

  Ibaraki Univ. Ibaraki Jpn

• Hayashi Takahisa

  Wood Research Institute, Kyoto University

• TAKEDA Takumi

  Wood Research Institute, Kyoto University

• MITSUISHI Yasushi

  National Institute of Bioscience and Human-Technology

• Mitsuishi Yasushi

  National Institute Of Bioscience And Human-technology Aist

• Mitsuishi Yasushi

  National Institute Of Bioscience And Human Technology

• Ogawa Kozo

  Resarch Institute for Advanced Sciencea and Technology, University of Osaka Prefecture

• Hayashi T

  Wood Research Institute Kyoto University

• Takeda Takumi

  Wood Research Institute Kyoto University

• Takeda Takumi

  Wood Res. Inst. Kyoto Univ.

• Ogawa K

  Dep.bot. Fac.sci. Kyoto Univ.

• Hayashi Takahisa

  Wood Res. Inst. Kyoto Univ.

• Takeda T

  Dep. Of Advanced Bioscience Fac. Of Agriculture Kinki Univ.

• HAYASHI Takahisa

  Wood Res. Inst. , Kyoto University, Gokasho, Uji, Kyoto

• TAKEDA Takumi

  Wood Res. Inst. , Kyoto Univ.

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