Xyloglucan Endotransglycosylation in Suspension-cultured Poplar Cells
スポンサーリンク
概要
- 論文の詳細を見る
Xyloglucan endotransglycosylase activity was identified and defined by transfer of a part of xyloglucan to reduced xyloglucan heptasaccharide ([^3H]XXXGol) in an enzyme preparations from suspension-cultured poplar cells. Although the activity was distributed in buffer-soluble and buffer-insoluble fractions associated with cells and in the extracellular fraction, it was mostly recovered in the buffer-insoluble fraction, suggesting that the enzyme was bound to the cell wall. The affinity for acceptor XXXGol was increased at a higher concentration of donor xyloglucan with a constant V_<max>. The V_<max> for donor xyloglucan was increased at a higher concentration of the oligosaccharide without any change in affinity. These kinetic data suggest that the acceptor acts by combining with the enzyme independently of the donor. The velocity of the reaction decreased gradually as the heptasaccharide units was increased from two to four, suggesting that the xyloglucan endotransglycosylase reaction caused donor xyloglucan substantially to decrease in molecular size. The activity in buffer-soluble fraction was increased by ABA in auxin-starved cells, when cultured in MS medium containing various plant hormones. Nevertheless, the activity increased markedly at the exponential growth and decreased immediately at the stationary phase of cells in the presence of 2,4-D. The activity of xyloglucan endotransglycosylase is developmentally regulated during growth but is not directly induced by plant hormones.
- 社団法人日本農芸化学会の論文
- 1996-12-23
著者
-
Tada T
Dep. Of Advanced Bioscience Fac. Of Agriculture Kinki Univ.
-
Sakai Fukumi
Wood Research Institute, Kyoto University
-
Hayashi Takahisa
Wood Research Institute, Kyoto University
-
TAKEDA Takumi
Wood Research Institute, Kyoto University
-
MITSUISHI Yasushi
National Institute of Bioscience and Human-Technology
-
Sakai F
Kyoto Univ.
-
Mitsuishi Yasushi
National Institute Of Bioscience And Human-technology Aist
-
Mitsuishi Yasushi
National Institute Of Bioscience And Human Technology
-
Hayashi T
Wood Research Institute Kyoto University
-
Takeda Takumi
Wood Research Institute Kyoto University
-
Takeda Takumi
Wood Res. Inst. Kyoto Univ.
-
Sakai Fukumi
Wood Research Institute Kyoto University
-
Hayashi Takahisa
Wood Res. Inst. Kyoto Univ.
-
Takeda T
Dep. Of Advanced Bioscience Fac. Of Agriculture Kinki Univ.
-
HAYASHI Takahisa
Wood Res. Inst. , Kyoto University, Gokasho, Uji, Kyoto
-
TAKEDA Takumi
Wood Res. Inst. , Kyoto Univ.
関連論文
- Cloning and Sequencing of a cDNA for Poplar Endo-1,4-β-Glucanase : GROWTH AND DEVELOPMENT : GENE STRUCTURE AND EXPRESSION
- Purification and Properties of a Wall-Bound Endo-1,4-β-Glucanase from Suspension-Cultured Poplar Cells : PROTEINS, ENZYMES AND METABOLISM
- Endo-1,4-β-glucanase in Suspension-cultured Poplar Cells
- RESISTANCE TO ENVIRONMENTAL STRESS IN TRANSGENIC TOBACCO OVEREXPRESSING OF CATALASE FROM E. coli IN CHLOROPLASTS
- Structural Analysis of Xyloglucan Oligosaccharides by the Post-source Decay Fragmentation Method of MALDI-TOF Mass Spectrometry : Influence of the Degree of Substitution by Branched Galactose, Xylose, and Fucose on the Fragment Ion Intensities
- Post-source Decay Fragment Analysis of a Highly Branched Nonasaccharide of Xyloglucan Using MALDI-TOF Mass Spectrometry
- Structural Analyses of Xyloglucan Heptasaccharide by the Post-source Decay Fragment Method Using Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
- Characterization of Rice Functional Monosaccharide Transporter, OsMST5(Biochemistry & Molecular Biology)
- Sugar transporters involved in flowering and grain development of rice
- Molecular characterization of sugar transporters involved in flowering and grain development of rice
- Purification of Xyloglucanase from Auxin-treated Pea Stems
- Synthesis of 13-Oxo-(Z)-9-octadecenoic Acid and 15-Oxo-(Z)-11-icosenoic Acid, Arrestants of Oryzaephilus surinamensis L.
- Arrestants to Oryzaephilus surinamensis L. from Wheat Flour Infested by the Same Weevil
- Lignan production in Daphne odora cell cultures
- Formation of callose from sucrose in cotton fiber microsomal membranes
- An Increase in Apparent Affinity for Sucrose of Mung Bean Sucrose Synthase Is Caused by In Vitro Phoshorylation or Directed Mutagenesis of Ser^
- CALLOSE BIOSYNTHESIS FROM SUCROSE IN COTTON MEMBRANES
- Synthesis of Asymmetrically Labeled Sucrose by a Recombinant Sucrose Synthase
- A Beta-Glucosidase Gene Downstream of the Cellulose Synthase Operon in Cellulose-producing Acetobacter
- Expression and Characterization of Sucrose Synthase from Mung Bean Seedlings in Escherichia coli
- Genomic DNA Cloning of Poplar Endo-1, 4-β-Glucanase
- PROPERTIES OF RECOMBINANT SUCROSE SYNTHASE
- EXPRESSION OF SUCROSE SYNTHASE CDNA AND ITS ACTIVITY IN ESCHERICHIA COLI
- Expression Analysis of Endo-1,4-β-Glucanase Genes, PopCel1 and PopCel2 in Poplar Cells
- Expression of Cellulases in Poplar Cells
- Analysis of the Oligosaccharide Units of Xyloglucans by Digestion with Isoprimeverose-producing Oligoxyloglucan Hydrolase Followed by Anion-exchange Chromatography
- Structural Analysis of the Solanaceae Xyloglucans Using Xyloglucanase Hydrolysis Method
- Characterization of Cross-Links between Cellulose Microfibrils, and Their Occurrence during Elongation Growth in Pea Epicotyl
- Analysis of the xyloglucan cross-links in the cell wall of pea
- ISOLATION OF XYLOGLUCAN SYNTHASE
- Xyloglucan Endotransglycosylation in Suspension-cultured Poplar Cells
- Characterization of Xyloglucan Endotransglycosylation in Suspension-cultured Poplar Cells
- Characterization of the adsorption of Xyloglucan to Cellulose : STRUCTURE AND FUNCTION OF CELLS
- Effects of the Degree of Polymerization on the Binding of Xyloglucans to Cellulose : STRUCTURE AND FUNCTION OF CELLS
- FUNCTIONAL ANALYSIS OF CELLULOSE-SYNTHASE-LIKE GENES
- COMPARISON OF REGULATION MECHANISM OFTHIOL ENZYMES INVOLVED IN THE PHOTOSYNTHETIC CAARBON REDUCTIONCYCLE BETWEEN ALGAE AND HIGHER PLANT
- Structural Basis for Thermostability of Endo-1,5-α-L-Arabinanase form Bacillus thermodenitrificans TS-3
- Feedback Inhibition of Spinach L-Galactose Dehydrogenase by L-Ascorbate
- Crystal Structure of Chloroplastic Ascorbate Peroxidase from Tobacco Plants and Structural Insights into its Instability
- Characterization of Ascorbate Peroxidases from Unicellular Red Alga Galdieria partita
- REGULATORY MECHANISMS OF THE CALVIN CYCLE IN CYANOBACTERIA
- THE ROLE OF SELENIUM FOR THE INDUCTION OF GLUTATHIONE PEROXIDASE IN Chlamydomonas reinhardtii
- Molecular Characterization of Tobacco Mitochondrial L-Galactono-γ-Lactone Dehydrogenase and Its Expression in Escherichia coli
- ANALYSIS OF PHYSIOLOGICAL FUNCTIONS OF CATALASE-PEROXIDASE IN Synechococcus PCC 7942 USING GENE-INTERRUPTING MUTANT
- Functional Analysis of Fructose-1, 6-Bisphosphatase Isozymes (fbp-I and fbp-II Gene Products) in Cyanobacteria
- GENE REGULATION OF CHLOROPLASTIC ASCORBATE PEROXIDASE ISOZYMES BY ALTERNATIVE mRNA SPLICING OF 3'-TERMINAL EXONS IN SPINACH
- Lack of Ligth/Dark Regulation of Enzymes Involved in the Photosynthetic Carbon Reduction Cycle in Cyanobacteria, Synechococcus PCC 7942 and Synechocystis PCC 6803
- Molecular Characterization and Physiological Role of a Glyoxysome-Bound Ascorbate Peroxidase from Spinach
- THE SPINACH CHLOROPLASTIC ASCORBATE PEROXIDASE GENE PRODUCES mRNAs FOR ISOZYMES BY ALTERNATIVE SPLICING AT 3'-TERMINAL EXONS
- MOLECULAR CHARACTERIZATION AND PHYSIOLOGICAL ROLE OF A MICROBODY-BOUND ASCORBATE PEROXIDASE FROM SPINACH
- THE MOLECULAR CHARACTERIZATION OF A NEW TYPE OF FRUCTOSE-1, 6-BISPHOSPHATASE IN Synechocystis PCC6803
- DEGRADATION OF PLANT CELL WALLS BYVARIOUS ENDO-1, 4-β-GLUCANASES
- Localization of Xyloglucan in the Macromolecular Complex Composed of Xyloglucan and Cellulose in Pea Stems : STRUCTURE AND FUNCTION OF CELLS
- CLONING OF cDNA INVOLVING TENSION WOODFORMATION
- Changes in Levels of mRNAs for Cell Wall-Related Enzymes in Growing Cotton Fiber Cells
- Expression System for Foreign Genes using Protoplasts from Liriodendron tulipifera L. Suspension Culture
- BIODEGRADATION OF CELLULOSE IN SUSPENSION-CULTURED POPLAR CELLS
- Transient Expression of Goat Growth Hormone Gene in Poplar (Populus alba L.) Protoplasts : A Quick Method for Detection of Foreign Gene Expression in mRNA Level
- ARRANGEMENT OF CORTICAL MICROTUBULES AND β-GLUCAN SYNTHASES IN PLASMA MEMBRANE GHOSTS OF TOBACCO BY-2 CELLS
- CLONING AND SEQUENCE ANALYSIS OF A cDNA ENCODING CHLOROPLASTIC ASCORBATE PEROXIDASE FROM SPINACH
- CLONING AND CHARACTERIZATION OF 1.2kbp-cDNA ENCODING SPINACH PEROXIDASE
- An O-Methyltransferase (OMT) cDNA Clone in Japanese Red Pine (Pinus densiflora) Seedlings
- Changes in Levels of mRNAs for Cell Wall-related Enzymes in Growing Cotton Cells
- GENE EXPRESSION OF WALL-MODIFYING ENZYMES IN THE GROWING COTTON FIBER CELLS
- ENZYMATIC AND MOLECULAR CHARACTERIZATIONOF CATALASE IN Synechococcus PCC 7942
- CHARACTERIZATION OF ASCORBATE PEROXIDASEFROM EUGLENA GRACILIS Z
- THE MOLECULAR CHARACTERIZATION OFH_2 O_2-RESISTANCE SYSTEM OF FRUCTOSE-1,6-BISPHOSPHATASE IN Synechococcus PCC7942
- ENZYMOLOGICAL AND MOLECULARCHARACTERIZATION OF NADP-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASEFROM Synechococcus PCC7942
- The Presence of Enzymes Related to Glutathione Metabolism and Oxygen Metabolism in Chlamydomonas reinhardtii
- Transferase activity of GhCesA2 (putative cotton cellulose 4-β-glucosyltransferase) expressed in Pichia pastoris
- A Homologue of EGL1 Encoding Endo-1,4-β-glucanase in Elongating Pea Stems
- Involvement of Xyloglucan Endotransglycosylase in Cellulose Biosynthesis
- OVEREXPRESSION OF POPLAR ENDO-1,4-β-GLUCANASE IN ARABIDOPSIS
- Cloning of Cotton Homologs of bcsA Gene Encoding Cellulose β-1,4-Glucosyltransferase
- LOCALIZATION OF pcsA POLYPEPTIDE IN COTTON CELLS
- Cloning and Sequencing of Cotton Homologs of bcsA Gene Encoding Cellulose 4-β-Glucosyltransferase
- Expression of Gene Encoding Endo-1, 4-β-glucanase in Suspension-cultured Poplar (Poplus alba L.) Cells
- The Contribution of Arabidopsis Homologs of L-Gulono-1,4-lactone Oxidase to the Biosynthesis of Ascorbic Acid
- CHANGES OF GENE EXPRESSION INVOLVING TENSION WOOD FORMATION
- キシログルカンに特異的な酵素を用いたキシログルカンの構造解析
- Compositional Analysis of the Oligosaccharide Units of Xyloglucans from Suspension-cultured Poplar Cells
- EFFECT OF IRON ON THE EXPRESSION OF ASCORBATE PEROXIDASE IN EUGLENA
- Simultaneous expression of stilbene synthase genes in Japanese red pine (Pinus densiflora) seedlings
- Characterization of Stilbene Synthase Genes in Japanese Red Pine (Pinus densiflora)
- MOLECULAR CHARACTERIZATION OF FRUCTOSE-1, 6-BISPHOSPHATASE ISOZYMES FROM Synechococcus PCC7942
- Enzymic and molecular characterization of NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Synechococcus PCC7942:resistance of the enzyme to hydrogen peroxide
- The catalase-peroxidase of Synechococcus PCC7942:purification,nucleotide sequence analysis and expression in Escherichia coli
- Subcellular Localization and Properties of L-Galactono-γ-lactone Dehydrogenase in Spinach Leaves
- GENE EXPRESSION OF ENDO-1, 4-β-GLUCANASE, ENDOXYLOGLUCAN TRANSFERASE AND EXPANSIN IN PEA STEMS
- EXPRESSION OFENDO-1, 4-β-GLUCANASE INSUSPENSION CULTURED POPLAR CELLS
- Production of Endo-1,4-β-glucanase Activity: A Simple Method for Suspension Culture of Poplar Cells
- Purification and Properties of an Extracellular Endo-1,4-β-Glucanase from Suspension-Cultured Poplar Cells
- Macromolecular Complexes of Xyloglucan and Cellulose Obtained by Annealing : STRUCTURE AND FUNCTION OF CELLS
- Carbohydrate Analysis of an Interspecific Hybrid between Onion and Garlic
- The Oligosaccharide Units of the Xyloglucans in the Cell Walls of Bulbs of Onion, Garlic and their Hybrid : STRUCTURE AND FUNCTION OF CELLS
- Relation between developmental changes on anatomical structure and on protein pattern in differentiating xylem of tension wood
- Comparison of three Chlamydomonas strains which show distinctive oxidative stress tolerance(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Resistance of Photosynthesis to Hydrogen Peroxide in Algae : ENVIRONMENTAL AND STRESS RESPONSES : PROTEINS, ENZYMES AND METABOLISM
- XYLOGLUCAN STORY IN THE APOPLAST
- Sugar transporters involved in flowering and grain development of rice