Intra- and Extra-Cellular Localization of "Cytosolic" CuZn-Superoxide Dismutase in Spinach Leaf and Hypocotyl

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概要

• 論文の詳細を見る

• Immunogold-electron microscopic analysis of spinach leaves done with the antibody specific for "cytosolic" CuZn-superoxide dismutase (SOD) indicates that SOD is localized in the apoplast, in the nucleus and in, or near, the tonoplast. The association of CuZn-SOD with the nucleus indicates it has a role in preventing fatal mutation caused by reactive species of oxygen. The localizing site of CuZn-SOD in the apoplastic region of spinach leaf tissues corresponds to that of the accumulation of lignin. In spinach hypocotyl "cytosolic" CuZn-SOD is localized in vascular tissues where lignification and the generation of superoxide respectively were shown by the phloroglucin-HCI reaction and formation of formazane from nitroblue tetrazolium. Because hydrogen peroxide is required for lignification via the peroxidase-catalyzed reaction, the CuZn-SOD in the apoplast appears to function in the biosynthesis of lignin by causing rapid disproportionation of the superoxide anion radical prior to its interaction with cellular components and peroxidase.

• 日本植物生理学会の論文

著者

• Asada Kozi

  Dept. Biotech Fac. Engin. Fukuyama Univ.

• Asada K

  The Research Institute For Food Science Kyoto University

• Asada Kozi

  The Research Institute for Food Science, Kyoto University

• Kanematsu Sumio

  Department of Food Science and Technology, Minami-Kyushu University

• Kanematsu Sumio

  Dept.food Sci.technol. Minami-kyushu Univ.

• Kanematsu S

  Minami‐kyushu Univ. Miyazaki Jpn

• Kanematsu Sumio

  Department Of Food Science And Technology Minami-kyushu University

• Ogawa Kenichi

  Department of Botany, Faculty of Science, Kyoto University

• Asada Kozi

  The Research Institute For Food Science Kyoto University:(present)department Of Biotechnology Facult

• Ogawa Kenichi

  Seiko Instruments Inc.

• Ogawa Ken'ichi

  Dep.bot. Fac.sci. Kyoto Univ.

• Asada K

  Dept. Biotech Fac. Engin. Fukuyama Univ.

• Asada Kozi

  The Research Institute For Food Science Kyoto Niversity

• Ogawa K

  Dep.bot. Fac.sci. Kyoto Univ.

• Ogawa Kenichi

  Dep.bot. Fac.sci. Kyoto Univ.

• Asada Kozi

  The Research Institulefor Fbod Science Kyoto University

• Ogawa Ken-ichi

  Dep.bot. Fac.sci. Kyoto Univ.

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