Occurrence of Cu,Zn-Superoxide Dismutase in the Intrathylakoid Space of Spinach Chloroplasts
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概要
- 論文の詳細を見る
Thylakoids obtained from intact spinach chloroplasts showed no superoxide dismutase (SOD) activity, but Cu,Zn- and Mn-SOD activities were detected in the presence of Triton X-100. Thylakoid membranes and the lumen fraction were separated by centrifugation after treatment of the thylakoids with a Yeda pressure cell. Cu,Zn-SOD was found in the lumen fraction. Mn-SOD was detected in the thylakoid fraction only after addition of 1 % Triton X-100. Antibody against spinach Cu,Zn-SOD did not interact with the latent Cu,Zn-SOD in the thylakoids unless Triton was added. These results indicate that Cu,Zn-SOD occurs in the lumen in addition to the stroma of spinach chloroplasts, and Mn-SOD binds to the thylakoid membranes.
- 日本植物生理学会の論文
著者
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Asada Kozi
The Research Institute for Food Science, Kyoto University
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Kanematsu Sumio
Department of Food Science and Technology, Minami-Kyushu University
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Kanematsu Sumio
Dept.food Sci.technol. Minami-kyushu Univ.
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Kanematsu S
Minami‐kyushu Univ. Miyazaki Jpn
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Kanematsu Sumio
Department Of Food Science And Technology Minami-kyushu University
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Kanematsu Sumio
The Research Institute For Food Science Kyoto University
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Hayakawa Takahiko
The Research Institute for Food Science, Kyoto University
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Hayakawa Takahiko
The Research Institute For Food Science Kyoto University
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Asada K
Dept. Biotech Fac. Engin. Fukuyama Univ.
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Asada Kozi
The Research Institute For Food Science Kyoto Niversity
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Asada Kozi
The Research Institulefor Fbod Science Kyoto University
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