Isolation of a Novel NAD(P)H-Quinone Oxidoreductase from the Cyanobacterium Synechocystis PCC6803
スポンサーリンク
概要
- 論文の詳細を見る
A novel NAD(P)H-quinone oxidoreductase (NQR) was isolated from the cyanobacterium Synechocystis PCC6803 by ion-exchange, affinity and gel-filtration chromatographies. Isolated NQR was found to be a drgA gene product that was a homodimer composed of 23-kDa subunits. It showed NAD(P)H-plastoquinone oxidoreductase activity with K_m values for NADPH and NADH of 12 and 48 μM, respectively. The activity was inhibited by thiolmodifying reagents, but not by rotenone, amobarbital, salicylhydroxamic acid, dicumarol, flavone, or diphenyleneiodonium chloride. Therefore, the Cys-147 residue is probably involved in the catalytic reaction. The amino acid sequence of the purified NQR had some homology with those of NADH oxidase, NAD(P)H-flavin oxidoreductase, and nitroreductase but did not contain either an adenine-binding motif or a phosphate-binding motif, thus, it is a new type of NQR.
- 日本植物生理学会の論文
著者
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Matsuo Michinori
Research Institute for Food Science, Kyoto University
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Endo Tsuyoshi
Research Institute for Food Science, Kyoto University
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Asada Kozi
Research Institute for Food Science, Kyoto University
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Matsuo Michinori
Research Institute For Food Science Kyoto University:(present Address)division Of Applied Life Scien
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Asada K
Fukuyama Univ. Hiroshima Jpn
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Asada Kozi
Dept. Biotech Fac. Engin. Fukuyama Univ.
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Asada Kozi
Faculty Of Bioscience And Biotechnology Fukuyama University
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Asada K
The Research Institute For Food Science Kyoto University
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ENDO Tsuyoshi
Kyoto Univ.Food Sci.
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Asada Kozi
The Research Institute For Food Science Kyoto University:(present)department Of Biotechnology Facult
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Endo Tsuyoshi
Department Of Bioresource Science Obihiro University Of Agriculture And Veterinary Medicine
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Endo Tsuyoshi
Department Of Plant Genes And Totipotency Graduate School Of Biostudies Kyoto University
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Asada K
Dept. Biotech Fac. Engin. Fukuyama Univ.
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Asada Kozi
Research Institute For Food Science Kyoto University
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Matsuo Masahiko
Research Institute For Food Science Kyoto University:(present Address)division Of Applied Life Scien
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Endo T
Graduate School Of Biostudies Kyoto University
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Asada Kozu
The Research Institute For Food Science Kyoto University
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