Increase of Cinnamaldehyde Groups in Lignin of Transgenic Tobacco Plants Carrying an Antisense Gene for Cinnamyl Alcohol Dehydrogenase

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概要

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• An antisense gene for Aralia cordata cinnamyl alcohol dehydrogenase (CAD) was introduced into tobacco plants. Two transgenic plants showed 55 and 20% reduction of the CAD activity compared to that of the control plant. Lignin content measured by the acetyl bromide method showed no significant differences between these plants and the control plant. However, the content of p-hydroxycinnamaldehyde groups in lignin was higher in the transgenic than in the control plants. The increase of p-hydroxycinnamaldehyde groups correlated inversely with the levels of CAD activity. Phloroglucinol staining of transverse sections of stem internode indicated that the fiber secondary wall reacted more strongly with the reagent in the transgenic plants. These results suggested that suppression of CAD activity in tobacco plants results in an increase of cinnamaldehyde groups in lignin, but no significant change in lignin content.

• 社団法人日本農芸化学会の論文
• 1995-05-23

著者

• Hibino Takashi

  Graduate School Of Environmental And Human Sciences Meijo University

• TAKABE Keiji

  Department of Wood Science and Technology, Faculty of Agriculture, Kyoto University

• Hibino T

  Meijo Univ. Aichi Jpn

• SHIBATA Daisuke

  Mitsui Plant Biotechnology Research Institute

• Hibino Takashi

  Mitsui Plant Biotechnology Research Institute

• Shibata Daisuke

  Graduate School Of Life Sciences Tohoku University

• Takabe Keiji

  Department Of Wood Science & Technology Faculty Of Agriculture

• Kawazu Tetsu

  Kameyama Breeding Station New Oji Institute For Forest Tree Improvement New Oji Paper Co. Ltd.

• Higuchi Takayoshi

  Department of Forestry, College of Agriculture and Veterinary Medicine, Nihon University

• Shibata Daisuke

  Mitsui Plant Biotech. Res. Inst.

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