Root specific expression of Na^+/H^+ antiporter gene from Synechocystis sp. PCC 6803 confers salt tolerance of tobacco plant

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概要

• 論文の詳細を見る

• Tobacco plants were transformed with a Nhap type Na+/H+ antiporter gene, SynnhaP1 (slr1595), from a cyanobacterium Synechocystis sp. PCC 6803. Two kinds of promoters, Arabidopsis alcohol dehydrogenase gene promoter (Adh promoter) and CaMV 35S promoter (35S promoter), were used. The transgenic plants driven by Adh promoter accumulated SynNhaP1 proteins only in root whereas the transgenic plants driven by 35S promoter accumulated SynNhaP1 proteins in all tissues. Confocal imaging of SynNhaP1-GFP fusion protein suggests the intracellular localization of SynNhaP1 in plasma membrane. Transgenic plants exhibited higher germination yields, increased biomass during developmental stage, increased seed production, and decreased intracellular Na+ content under salt-stress conditions. The transgenic plants driven by Adh promoter exhibited similar or slightly higher salt tolerance than that by 35S promoter. These results indicate the importance of expression of Na+/H+ antiporter in root for salt tolerance in plant.

• 日本植物細胞分子生物学会の論文
• 2006-06-01

著者

• 高倍 昭洋

  名城大学総合学術研究科

• Hibino Takashi

  Graduate School Of Environmental And Human Sciences Meijo University

• Tanaka Y

  Graduate School Of Environmental And Human Sciences Meijo University

• Takabe Teruhiro

  Research Institute, Meijo University

• Hibino T

  Graduate School Of Environmental And Human Sciences Meijo University

• Hibino T

  Meijo Univ. Aichi Jpn

• Hibino Takashi

  Institute Of Science Meijo University

• HOSSAIN Gazi

  Graduate School of Environmental and Human Sciences, Meijo University

• WADITEE Rungaroon

  Research Institute, Meijo University

• TANAKA Yoshito

  Graduate School of Environmental and Human Sciences, Meijo University

• Takabe Teruhiro

  Research Institute Meijo University

• Takabe T

  Res. Inst. Meijo Univ.

• Takabe Tetsuko

  Bioscience Center Nagoya University

• Hossain Gazi

  Graduate School Of Environmental And Human Sciences Meijo University

• Tanaka Yoshito

  Graduate School Of Environmental And Human Sciences Meijo University

• Higuchi Tatsuji

  Laboratory Of Biochemistry Graduate School Of Bioagricultural Sciences Nagoya University

• Higuchi Takashi

  Plant Molecular Physiology Research Institute Of Innovative Technology For The Earth (rite)

• Waditee Rungaroon

  Research Institute Meijo University

• Tanaka Yoshito

  Dept. Chem. Fac. Sci. And Tech. Meijo Univ.

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