Immunological Studies of Betaine Aldehyde Dehydrogenase in Barley : ENVIRONMENTAL AND STRESS RESPONSES

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概要

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• The changes in the level of the protein for betaine aldehyde dehydrogenase, which catalyzes the last step in the synthesis of glycinebetaine, were analyzed with antiserum raised against SDS-denatured betaine aldehyde dehydrogenase from spinach. In barley leaves, the levels of betaine aldehyde dehydrogenase protein were found to be enhanced by the addition of 200 mM NaCl to the growth medium. These changes in the level of the enzyme protein corresponded to those in the activity of the enzyme, as described in our previous study (Arakawa et al. 1990). The extent of this enhancement was reduced when barley plants were relieved from salt stress. An increase in the level of the protein was also induced by water stress, such as the withholding of water or the addition of polyethylene glycol 6000. Betaine aldehyde dehydrogenase protein was detected in etiolated leaves and roots, as well as in green leaves. In etiolated leaves, the level of betaine aldehyde dehydrogenase protein was not affected by salt stress.

• 日本植物生理学会の論文

著者

• Arakawa Keita

  Inst. Low Temp. Sci. Hokkaido Univ.

• Arakawa Keita

  Research Institute For Biochemical Regulation School Of Agricultural Science Nagoya University

• KISHITANI Sachie

  Laboratory of Plant Breeding and Genetics, Graduate School of Agricultural Science, Tohoku Universit

• Takabe T

  Res. Inst. Meijo Univ.

• Takabe Tetsuko

  Research Institute For Biochemical Regulation School Of Agriculture Nagoya University

• Arakawa Keita

  The Institute Of Low Temperature Science Hokkaido University

• Kishitani S

  Tohoku Univ. Sendai Jpn

• Kishitani Sachie

  Laboratory Of Plant Breeding And Genetics Graduate School Of Agricultural Science Tohoku University

• MIZUNO Katsuhiko

  Research Institute for Biochemical Regulation, School of Agricultural Science, Nagoya University

• Takabe T

  Research Institute For Biochemical Regulation School Of Agricultural Science Nagoya University

• Mizuno K

  Research Institute For Biochemical Regulation School Of Agricultural Science Nagoya University

• ARAKAWA Keita

  Research Faculty and Graduate School of Agriculture, Hokkaido University

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