Effect of oxygen on photosynthesis and biosynthesis of glycolate in photoheterotrophically grown cells of Rhodospirillum rubrum
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概要
- 論文の詳細を見る
Photosynthetic CO_2 fixation was studied using cells of Rhodospirillum rubrum grown heterotrophically on malate or butyrate. Rates of CO_2 fixation were higher in the malate-grown cells than in the butyrate-grown bacteria but ribulosebisphosphate (RuP_2) carboxylase/oxygenase activities were higher in the extracts prepared from the butyrate-grown bacteria. The photosynthetic CO_2 fixation in the butyrate-grown R. rubrum cells was inhibited by KCN, and the inhibitory effect of O_2 on CO_2 fixation was reversed when cells were returned to an N_2 atmosphere. In the malate-grown cells, photosynthetic CO_2 fixation was insensitive to KCN and the inhibitory effect exerted by O_2 was practically irreversible. ^<14>CO_2 was not incorporated into glycolate by either malate- or butyrate-grown cells in an N_2 atmosphere, but small amounts of labeled glycolate were found in malate- and butyrate-grown cells in air or 100% O_2. Glycolate excreted by these cells in 100% O_2 was measured colorimetrically and its identity established by mass spectrometry. When the O_2 atmosphere was labeled with ^<18>O_2, only one of the carboxyl oxygens of the excreted glycolate was labeled, and the enrichment of ^<18>O in this carboxyl oxygen relative to the ^<18>O_2 provided was greater than 80%. These studies show that significant glycolate production by R. rubrum only occurs in the presence of O_2 and that in both malateand butyrate-grown cells, the glycolate so formed is presumably produced via RuP_2 oxygenase.
- 日本植物生理学会の論文
著者
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Akazawa T
Research Institute For Biochemical Regulation School Of Agriculture
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Takabe Tetsuko
Research Institute For Biochemical Regulation School Of Agriculture Nagoya University
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Osmond.b C
Research Institute For Biochemical Regulation School Of Agriculture Nagoya University:department Of
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Summons.E R
Department of Environmental Biology, Research School of Biological Sciences, Australian National Uni
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Summons.e R
Department Of Environmental Biology Research School Of Biological Sciences Australian National Unive
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Akazawa T
Research Institute For Biochemical Regulation School Ofagriculture Nagoya University
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