Biosynthetic mechanism of glycolate in Chromatium IV. Glycolate-glycine transformation
スポンサーリンク
概要
- 論文の詳細を見る
The metabolic transformation of glycolate to glycine occurring in photosynthesizing cells of Chromatium was investigated by the radioisotopic technique and by amino acid analysis. By analyzing the distribution of radiocarbon upon feeding [1-^<14>C] glycolate, [2-^<14>C] glyoxylate and [1-^<14>C] glycine to bacterial cells, it was demonstrated that glycolate is converted to glycine via glyoxylate, and both glycolate and glycine are excreted extracellularly. Although the formation of serine was barely detected by the above two techniques in both N_2 and O_2 atmospheres, it was found that ^<14>CO_2 is evolved quite markedly from both [1-^<14>C] glycolate and [1-^<14>C] glycine fed to the Chromatium cells. Analytical results of transient changes in amino acid compositions under atmospheric changes of N_2→O_2 and by the addition of exogenous glycolate in N_2 confirm the notion that glycolate is converted to glycine. Acidic amino acids (glutamic acid and aspartic acid) appear to take part in glycine formation as amino donors. The formation of glycine from glycolate in a N_2 atmosphere suggests that an unknown glycolate dehydrogenation reaction may operate in the overall process.
- 日本植物生理学会の論文
著者
-
Akazawa Takashi
Research Institute for Biochemical Regulation, School of Agriculture, Nagoya University
-
Takabe Tetsuko
Research Institute For Biochemical Regulation School Of Agriculture Nagoya University
-
Asami Sumio
Research Insititute For Biochemical Regulation Nagoya University School Of Agriculture
-
Akazawa Takashi
Research Insititute For Biochemical Regulation Nagoya University School Of Agriculture
関連論文
- Purification and Characterization of Glyoxysomal Enzymes from Germinating Pumpkin Cotyledons
- Distinct Profiles of ADP-and UDP-Specific Sucrose Synthases in Developing Rice Grains
- Isolation and Characterization of Nuclei from Rice Embryos
- Determination of Intracellular Chloride Ion Concentration in a Halotolerant Cyanobacterium Aphanothece Halophytica
- Betaine Aldehyde Dehydrogenase in the Gramineae : Levels in Leaves of Both Betaine-Accumulating and Nonaccumulating Cereal Plants
- Immunological Studies of Betaine Aldehyde Dehydrogenase in Barley : ENVIRONMENTAL AND STRESS RESPONSES
- Polypeptide Compositions of Stroma, Internal Membranes, and Inner and Outer Envelope Membranes of Amyloplast from Suspension-Cultured Cells of Sycamore (Acer pseudoplatanus L.)
- Molecular evolution of ribulose-1,5-bisphosphate carboxylase
- Adenylate Uptake by Proplastids from Cultured Cells of Tobacco (Nicotiana tabacum L. cv. BY2) Indicates That an Adenylate Translocator is Present in All Types of Plastid
- A Method for Accurate Analysis of Intermembrane Space in Organelles Enclosed by Double Envelope Membranes
- 1Ba01 DNA Methylation as a Regulatory Mechanism of Photosynthesis Gene Expression in Non-photosynthetic Cells
- Ribulose-1,5-bisphosphate carboxylase in the bundle sheath cells of maize leaf
- Isolation and Characterization of Golgi Membranes from Suspension-Cultured Cells of Sycamore (Acer pseudoplatanus L.)
- Distribution of 59-and 55-kDa Catalase in Dark- and Light-grown Pumpkin and Various Other Plant Tissues
- Effect of α-hydroxy-2-pyridinemethanesulfonate on glycolate metabolism in spinach leaf protoplasts
- Levels of Betaine and Betaine Aldehyde Dehydrogenase Activity in the Green Leaves, and Etiolated Leaves and Roots of Barley
- Formation of Chlorophyll-Protein Complexes during Greening 1. Distribution of Newly Synthesized Chlorophyll among Apoproteins
- Glycogen biosynthesis in Chromatium strain D II. Purification and properties of glycogen synthetase
- Biosynthetic mechanism of glycolate in Chromatium I. Glycolate pathway
- Oxidative formation of glycolic acid in photosynthesizing cells of Chromatium
- Biosynthetic mechanism of glycolate in Chromatium IV. Glycolate-glycine transformation
- Biosynthetic mechanism of glycolate in Chromatium II. Enzymic mechanism of glycolate formation by a transketolase system
- Effect of oxygen on photosynthesis and biosynthesis of glycolate in photoheterotrophically grown cells of Rhodospirillum rubrum
- A comparative study on the effect of O_2 on photosynthetic carbon metabolism by Chlorobium thiosulfatophilum and Chromatium vinosum
- Biosynthetic mechanism of glycolate in Chromatium III. Effects of α-hydroxy-2-pyridinemethanesulfonate, glycidate and cyanide on glycolate excretion
- Biosynthetic mechanism of glycolate in Chromalium 6. Glycolate formation and metabolism under low O_2
- Substrate specificity of the granule-bound and chloroplastic starch synthetase