Biosynthetic mechanism of glycolate in Chromatium III. Effects of α-hydroxy-2-pyridinemethanesulfonate, glycidate and cyanide on glycolate excretion

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Effects of α-hydroxy-2-pyridinemethanesulfonate (α-HPMS), 2,3-epoxypropionate (glycidate), and cyanide on the photosynthetic activities of Chromatium were studied. α-HPMS stimulated photosynthetic CO_2 fixation in the bacterial cells in both N_2 and O_2 environments. The formation and subsequent excretion of both glycolate and glycine in the O_2 atmosphere were markedly enhanced by α-HPMS. In contrast to a recent report by Zelitch [Arch. Biochem. Biophys. 163 : 367-377 (1974)] that glycidate specifically inhibits glycolate formation in tobacco leaf disks, we found that it had no influence on CO_2 fixation by Chromatium in either N_2 or O_2 atmosphere, and that the synthesis and extracellular excretion of glycolate were markedly stimulated by glycidate treatment. Cyanide (0.01-1 mM) exerted a marked inhibitory effect on photosynthetic CO_2 fixation in N_2. In O_2 atmosphere, photosynthesis was stimulated by 0.01 mM cyanide, and inhibited by it above this level. Both the incorporation of ^<14>CO_2 into glycolate and the total synthesis of glycolate in the light were also enhanced by 0.01 mM cyanide, and strongly inhibited above that concentration.
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