Effect of α-hydroxy-2-pyridinemethanesulfonate on glycolate metabolism in spinach leaf protoplasts

概要

論文の詳細を見る
The addition of a-hydroxy-2-pyridinemethanesulfonate (α-HPMS) to spinach leaf protoplasts caused a marked inhibition of photosynthetic CO_2 fixation in both air and O_2 atmospheres. In the O_2 atmosphere ^<14>CO_2 was. incorporated into glycine, but upon addition of α-HPMS (10 mM), there was a suppression of ^<14>CO_2 incorporation into the glycine and serine plus isoleucine fractions, accompanied by an accumulation of ^<14>C-glycolate. A marked stimulation of alanine labeling due to the α-HPMS treatment was also observed. Feeding protoplasts with [1-^<14>C]-glycolate resulted in the formation of ^<14>C-labeled glycine, serine, and sugar phosphates in both light and dark conditions, and a sizable amount of ^<14>CO_2 evolved concomitantly. The results support the notion concerning the operation of the glycolate pathway in leaf tissue during photorespiratory environments. The suitability of protoplasts for photosynthetic research in conjunction with the use of inhibitor substances are discussed, although the results of the present research indicate that the effect of α-HPMS is not confined to the specific inhibition of the glycolate-oxidase reaction.
日本植物生理学会の論文