Biosynthetic mechanism of glycolate in Chromatium I. Glycolate pathway

概要

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The pattern of radioactivity distribution in several amino acids of Chromatium cells exposed to ^<14>CO_2 was determined. By transferring the bacterial cells from an atmosphere of nitrogen to oxygen there occurred a transient decrease of ^<14>CO_2 in-corporation into aspartate and glutamate, whereas that into glycine showed a prominent increase. The labeling of both serine and alanine did not show a marked change under such conditions. The activities of glycolate oxidase and glycolate dehydrogenase in crude extracts of the bacterial cells were very low. The formation of glycolic acid only occurred during the oxidative metabolism of Chromatium cells grown on bicarbonate as a C source, being negligibly small in bacteria under nitrogen or after growth on malate or acetate. The activities of both ribulose-1,5-bisphosphate oxygenase and phosphoglycolate phosphatase in the extract prepared from the bicarbonate-grown bacterial cells were very low and apparently could not account for the glycolic acid formation through these enzymic reactions. Metabolic patterns of glycolic acid in Chromatium are discussed in relation to the photorespiratory phenomenon.
日本植物生理学会の論文