Properties of Two N-Linked Glycoproteins Associated with the Nuclear Envelope in Mung Bean Hypocotyls : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
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概要
- 論文の詳細を見る
Nuclei from mung bean (Vigna radiata) hypocotyls contained two glycoproteins of 50 and 49 kDa, respectively, that reacted with concanavalin A. The glycoproteins were released from the nuclear envelope by treatment with 2 M KCl but not with nucleases. The glycoproteins, tentatively named gp50 and gp49, were isolated and characterized. Gel-permeation chromatography suggested that gp50 and gp49 seem to exist as a complex with other components. The glycoproteins could be detected only in the nuclear fraction by immunoblot analysis with specific antibodies, and they were not detected in endoplasmic reticulum, plasma membrane, vacuolar membrane or mitochondria. Agglutinin I from Ulex europeaus, peanut agglutinin, soybean agglutinin and wheat germ agglutinin all failed to bind to the glycoproteins. Treatment with glycopeptidase F removed all oligosaccharides from the glycoproteins and decreased their molecular masses by about one thousand daltons each. These results suggest that the glycoproteins contained N-linked, high-mannose-type oligosaccharides with six or seven hexose residues. gp50 and gp49 seemed to be isoforms of a single glycoprotein because the two proteins had some common properties. Nuclear fractions from azuki bean (Phaseolus angularis) and soybean (Glycine max) contained proteins that were immunologically similar to gp50 and gp49.
- 日本植物生理学会の論文
著者
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ARAKAWA Keita
Institute of Low Temperature Science, Hokkaido University
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YOSHIDA Shizuo
Institute of Low Temperature Science, Hokkaido University
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Maeshima Masayoshi
Graduate School of Bioagricultural Sciences, Nagoya University
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MAESHIMA Masayoshi
Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University
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Arakawa Keita
Inst. Low Temp. Sci. Hokkaido Univ.
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Arakawa Keita
Research Institute For Biochemical Regulation School Of Agricultural Science Nagoya University
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Arakawa Keita
Institute Of Low Temperature Science Hokkaido University
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ODAIRA Masato
Hokkaido National Industrial Resaerch Institute
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Maeshima Masayoshi
Laboratory Of Biochemistry School Of Agriculture Nagoya University:(present)institute Of Low Tempera
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Maeshima Masayoshi
School Agriculture Nagoya Univ.
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Maeshima Masayoshi
Department Of Agricultural Chemistry Nagoya University
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Maeshima Masayoshi
Laboratory Of Biochemistry Graduate School Of Bioagricultural Sciences Nagoya University
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Maeshima M
Laboratory Of Cell Dynamics Graduate School Of Bioagricultural Sciences Nagoya University
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Odaira Masato
Institute Of Low Temperature Science Hokkaido University:(present)bioscience And Chemistry Division
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Arakawa Keita
The Institute Of Low Temperature Science Hokkaido University
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Yoshida Shizuo
Institute Of Low Temperature Science Hokkaido University
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Maeshima Masayoshi
Laboratory Cell Dynamics Graduate School Of Bioagricultural Sciences Nagoya University
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