Immunochemical Analysis of Aquaporin Isoforms in Arabidopsis Suspension-Cultured Cells
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概要
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Aquaporins mediate the movement of water across biomembranes. Arabidopsis thaliana contains 35 aquaporins that belong to four subfamilies (PIP, TIP, SIP, and NIP). We investigated their expression profiles immunochemically in suspension-cultured Arabidopsis thaliana cells during growth and in response to salt and osmotic stresses. Protein amounts of all aquaporins were much lower in cultured cells than in the plant tissues. This is consistent with the low water permeability of protoplasts from cultured cells. After treatment with NaCl, the protein amounts of PIP2;1, PIP2;2, and PIP2;3 in the cells increased several-fold, and those of TIP1;1 and TIP1;2, 15- and 3-fold respectively. PIP1 did not change under the stress. Cell death began after 19 d in culture, accompanied by marked accumulation of PIPs and TIPs and a gradual decrease in SIPs. Our results suggest the followings: (i) Accumulation of aquaporin isoforms was individually regulated at low levels in single cells. (ii) At least PIP2;2, PIP2;3, TIP1;1, and TIP1;2 are stress-responsive aquaporins in suspension cells. (iii) A sudden increment of several members of PIP2 and TIP1 subfamilies might be related to cell death.
- 社団法人 日本農芸化学会の論文
- 2006-04-23
著者
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SEGAMI Shoji
Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University
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MAESHIMA Masayoshi
Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University
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Segami Shoji
Laboratory Of Cell Dynamics Graduate School Of Bioagricultural Sciences Nagoya University
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Kobae Yoshihiro
Laboratory Of Cell Dynamics Graduate School Of Bioagricultural Sciences Nagoya University
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MIZUTANI Masahiro
Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University
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Segami Shoji
Lab. Of Cell Dynamics Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Maeshima Masayoshi
Laboratory Cell Dynamics Graduate School Of Bioagricultural Sciences Nagoya University
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Mizutani Masahiro
Laboratory Of Cell Dynamics Graduate School Of Bioagricultural Sciences Nagoya University
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