Characterization of Proteins of Nuclear Envelopes Prepared from Mung Bean Hypocotyls : PROTEINS, ENZYMES AND METABOLISM
スポンサーリンク
概要
- 論文の詳細を見る
The polypeptide composition of nuclear envelopes prepared from hypocotyls of mung bean (Vigna radiata) was investigated. The tissue was homogenized in the presence of Triton X-100 and nuclei were isolated by differential and discontinuous Percoll gradient centrifugation. The nuclei were subjected to sonication in 2 M KCl or 50 mM lithium diiodosalicylate and then the nuclear envelopes were collected by centrifugation. Proteins in the envelope fraction were analyzed by sodium dodecylsulfate-polyacrylamide gel electrophoresis and blotting techniques. When the envelope fraction was incubated with [γ-^<32>P]ATP, 10 to 15 polypeptides were labeled and the intensity of labeling of some of these polypeptides was enhanced by the addition of calcium ions. The results suggest the presence of a protein-phosphorylation system in nuclear envelopes. Three polypeptide of 100, 42, and 40 kDa stained blue with the cationic carbocyanine dye "Stains-all" , and they were labeled with ^<45>Ca^<2+> on a transfer membrane. The lectin concanavalin A recognized glycoproteins that migrated as polypeptides of 50, 49, 47, 43, 35 and 32 kDa, respectively. Of these polypeptides the two larger ones were prominent and were solubilized by treatment of the envelope fraction with KCl at 2 M but not at less than 100 mM. These results suggest that the mung bean nuclear envelope contains some calcium-binding proteins and glycoproteins. These newly identified proteins may become useful as characteristic markers of the nuclear envelope.
- 日本植物生理学会の論文
著者
-
ODAIRA Masato
Hokkaido National Industrial Resaerch Institute
-
Odaira Masato
Institute Of Low Temperature Science Hokkaido University:(present)bioscience And Chemistry Division
-
Maeshima Masayoshi
Laboratory Of Biochemistry School Of Agricultural Sciences Nagoya University
-
Maeshima Masayoshi
Laboratory Cell Dynamics Graduate School Of Bioagricultural Sciences Nagoya University
関連論文
- Evidence for No Proteolytic Processing during Transport of Isocitrate Lyase into Glyoxysomes in Castor Bean Endosperm
- Molecular Cloning of Vacuolar H^+-pyrophosphatase and Its Expression during the Development of Pear Fruit
- Changes in H^+-Pumps and a Tonoplast Intrinsic Protein of Vacuolar Membranes during the Development of Pear Fruit
- PROPERTIES AND ROLES IN FRUIT DEVELOPMENTOF VACUOLAR H^+-ATPASE, H H^+-PYROPHOSPHATASE ANDVM-23 IN PEAR FRUIT
- p-COUMARIC ACID FROM WHEAT INACTIVATES ICE-NUCLEATING ACTIVITY BY ICE-NUCLEATING BACTERIUM
- Tissue-Specific Isoforms of Catalase Subunits in Castor Bean Seedlings
- Proton Pumps of the Vacuolar Membrane in Growing Plant Cells
- The Nuclear Gene for Subunit Vc of Sweet Potato Cytochrome c Oxidase
- Properties of the Dicyclohexylcarbodiimide-Binding Subunit of Cytochrome c Oxidase from Sweet Potato
- N-Terminal Amino Acid Sequence and Processing Site of Sweet Potato Cytochrome c Oxidase Subunit II
- Differences in the Thermostability and Subunit Species of Cytochrome c Oxidase among Tissues
- Suppression by Exogenous Phospholipid of Cyanide-Insensitive Respiration of Submitochondrial Particles from Sweet Potato Root Tissue
- Immunological Studies on ATPases in Mung Bean Hypocotyl Plasma Membrane : Proposal of the Presence of Two Molecular Species of ATPase
- Low Aquaporin Content and Low Osmotic Water Permeability of the Plasma and Vacuolar Membranes of a CAM Plant Gratopetalum paraguayense: Comparison with Radish
- Molecular Cloning, Water Channel Activity and Tissue Specific Expression of Two Isoforms of Radish Vacuolar Aquaporin
- Aquaporin Isoforms Responsive to Salt and Water Stresses and Phytohormones in Radish Seedlings
- Water Channel Activity of Radish Plasma Membrane Aquaporins Heterologously Expressed in Yeast and Their Modification by Site-Directed Mutagenesis
- Differences in Aquaporin Levels among Cell Types of Radish and Measurement of Osmotic Water Permeability of Individual Protoplasts
- Modulation of Vacuolar H^+-Pumps and Aquaporin by Phytohormones in Rice Seedling Leaf Sheaths(Biochemistry/Molecular Biology)
- Loss of AtPDR8, a Plasma Membrane ABC Transporter of Arabidopsis thaliana, Causes Hypersensitive Cell Death Upon Pathogen Infection
- Expression of Functional Streptomyces coelicolor H^+-Pyrophosphatase and Characterization of Its Molecular Properties
- Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized to Vacuolar Membranes and Implicated in Zinc Homeostasis
- Acylspermidine Derivatives Isolated from a Soft Coral, Sinularia sp., Inhibit Plant Vacuolar H^+-Pyrophosphatase
- Cycloprodigiosin Uncouples H^+-Pyrophosphatase of Plant Vacuolar Membranes in the Presence of Chloride Ion
- Analysis of the Substrate Binding Site and Carboxyl Terminal Region of Vacuolar H^+-Pyrophosphatase of Mung Bean with Peptide Antibodies
- Accumulation of a Glycoprotein That is Homologous to a Seed Storage Protein in Mung Bean Hypocotyls at the Late Stage of Tissue Elongation
- Properties of Two N-Linked Glycoproteins Associated with the Nuclear Envelope in Mung Bean Hypocotyls : PROTEINS, ENZYMES AND METABOLISM : MEMBRANES AND BIOENERGETICS
- The Activity of the Root Vacuolar H^+-Pyrophosphatase in Rye Plants Grown under Conditions Deficient in Mineral Nutrients
- Determination of the Solution Structure of the N-Domain Plus Linker of Antarctic Eel Pout Antifreeze Protein RD3
- Physiological and Biochemical Significance of Antifreeze Substances in Plants
- SEARCHING FOR NEW ANTIFREEZE SUBSTANCES FROM WHEAT
- Immunochemical Analysis of Aquaporin Isoforms in Arabidopsis Suspension-Cultured Cells
- A Mutant Strain Arabidopsis thaliana that Lacks Vacuolar Membrane Zinc Transporter MTP1 Revealed the Latent Tolerance to Excessive Zinc
- An Arabidopsis Hydrophilic Ca^-Binding Protein with a PEVK-Rich Domain, PCaP2, is Associated with the Plasma Membrane and Interacts with Calmodulin and Phosphatidylinositol Phosphates
- Aquaporin NIP2 ; 1 is Mainly Localized to the ER Membrane and Shows Root-Specific Accumulation in Arabidopsis thaliana
- RVCaB, a Calcium-binding Protein in Radish Vacuoles, is Predominantly an Unstructured Protein with a Polyproline Type II Helix
- A Plasma Membrane-associated Protein Arabidopsis thaliana AtPCaP1 Binds Copper Ions and Changes Its Higher Order Structure
- Effect of Low Root Temperature on Hydraulic Conductivity of Rice Plants and the Possible Role of Aquaporins
- Tissue and Cell-Specific Localization of Rice Aquaporins and Their Water Transport Activities
- Functional Enhancement by Single-residue Substitution of Streptomyces coelicolor A3(2) H^+-translocating Pyrophosphatase
- Vacuolar Proton Pumps and Aquaporins Involved in Rapid Internode Elongation of Deepwater Rice
- Expression of the Vacuolar Ca^/H^+ Exchanger, OsCAX1a, in Rice : Cell and Age Specificity of Expression, and Enhancement by Ca^
- Quantification, Organ-Specific Accumulation and Intracellular Localization of Type II H^+-Pyrophosphatase in Arabidopsis thaliana
- Expression Profile of the Genes for Rice Cation/H^+ Exchanger Family and Functional Analysis in Yeast
- Molecular Properties of a Matrix Attachment Region-Binding Protein Located in the Nucleoli of Tobacco Cells
- Transcriptional Induction of Two Genes for CCaPs, Novel Cytosolic Proteins, in Arabidopsis thaliana in the Dark
- Characterization of Proteins of Nuclear Envelopes Prepared from Mung Bean Hypocotyls : PROTEINS, ENZYMES AND METABOLISM
- Mechanism of the Increase in Catalase Activity through Microbody Development in Wounded Sweet Potato Root Tissue
- Quantification of Ca^/H^+ Antiporter VCAX1p in Vacuolar Membranes and Its Absence in Roots of Mung Bean
- The Subunit Composition of Sweet Potato Cytochrome b-c1 Complex