Modulation of Vacuolar H^+-Pumps and Aquaporin by Phytohormones in Rice Seedling Leaf Sheaths(Biochemistry/Molecular Biology)
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概要
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The effects of exogenously applied gibberellic acid (GA_3) or brassinolide (BL) on the H^+-pumps and aquaporin in the vacuolar membrane of rice seedling leaf sheath were investigated. Antibodies against mung bean H^+-PPase, the B subunit of V-ATPase, and radish tonoplast intrinsic protein (TIP) cross-reacted with the vacuolar membrane proteins of rice seedling leaf sheath. The results of immunoblot analysis showed that the amounts of H^+-PPase and V-ATPase were retained at a high level for two days in the presence of GA_3, although the level gradually decreased without phytohormones, indicating that GA_3 has a promotive effect on the activities of vacuolar H^+-pumps. However, the levels of V-ATPase and H^+-PPase were increased on day 2 and then decreased when treated with BL. There were no visible differences in the level of TIP upon treatment with GA_3 and BL. In comparison with the water control, the activity of H^+-PPase treated with BL was also retained at a relatively high level, suggesting that BL has a stimulative effect on the activities of H^+-PPase. These results indicate that GA_3 and BL might be involved in the regulation of the quantity and activities of plant vacuolar H^+-pumps.
- 公益社団法人日本薬学会の論文
- 2003-01-01
著者
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MAESHIMA Masayoshi
Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University
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Komatsu Setsuko
National Institute of Crop Science
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Tanaka Yoshiyuki
National Institute of Agrobiological Resources
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Maeshima Masayoshi
Laboratory Of Biochemistry Graduate School Of Bioagricultural Sciences Nagoya University
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Komatsu S
Department Of Molecular Genetics National Institute Of Agrobiological Sciences
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YANG Shihai
National Institute of Agrobiological Sciences
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Komatsu Setsuko
National Inst. Of Crop Sci.
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Tanaka Yoshiyuki
National Institute Of Agrobiological Sciences
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Maeshima Masayoshi
Laboratory Of Cell Dynamics Nagoya University Graduate School Of Bioagricultural Sciences
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Maeshima Masayoshi
Laboratory Cell Dynamics Graduate School Of Bioagricultural Sciences Nagoya University
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Tanaka Yoshiyuki
National Inst.of Agrobiol.res.
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