Ectopic expression of DnaK chaperone from a halotolerant cyanobacterium Aphanothece halophytica induced the bolting without cold treatment in Eustoma grandiflorum

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The DnaK/Hsp70 family is a molecular chaperone that binds non-native states of other proteins, and affects various physiological processes in bacterial, plant and animal cells. In this study, a dnaK gene from a halotolerant cyanobacterium, Aphanothece halophytica, was introduced into Eustoma grandiflorum. The ApdnaK transformed plants could grow at a similar rate with the control plants expressing the vector alone. Although exposure to low temperature is required for the bolting of control Eustoma, it was not required for the ApdnaK transformant. Under normal growth conditions, the glutathione content in the ApdnaK transformants was higher than that of control plants. In the transformant, the pistil is not evident and consequently, no seed formation. The results suggest the involvement of molecular chaperone for the bolting of Eustoma.
2010-12-25