Cinnamyl Alcohol Dehydrogenase from Aralia cordata : Cloning of the cDNA and Expression of the Gene in Lignified Tissues
スポンサーリンク
概要
- 論文の詳細を見る
A full-length cDNA clone encoding a subunit of cinnamyl alcohol dehydrogenase (CAD) was isolated from a perennial dicot, Aralia cordata. The identity of the clone was demonstrated by two criteria: (i) the amino acid sequences of peptides derived from the purified CAD protein of A. cordata were highly homologous to regions of the amino acid sequence deduced from the nucleotide sequence of the cDNA; and (ii) a fusion protein expressed from λgt11 that carried part of the cDNA reacted with an antibody raised against purified CAD protein. Amino-terminal sequencing of the protein indicated that the mature protein is shorter by two amino acids at the amino terminus than the protein predicted from the nucleotide sequence. The level of expression of the gene was higher in stems than in leaves and roots, consistent with the degree of lignification of these tissues. A comparison of amino acid sequences indicated that CAD is a member of the family of alcohol dehydrogenases.
- 日本植物生理学会の論文
著者
-
Hibino Takashi
Graduate School Of Environmental And Human Sciences Meijo University
-
Hibino T
Meijo Univ. Aichi Jpn
-
SHIBATA Daisuke
Mitsui Plant Biotechnology Research Institute
-
Hibino Takashi
Mitsui Plant Biotechnology Research Institute
-
Shibata Daisuke
Graduate School Of Life Sciences Tohoku University
-
Shibata Daisuke
Mitsui Plant Biotech.res.inst.
-
Chen Jing-qing
Mitsui Plant Biotechnology Research Institute
-
Higuchi Takayoshi
Department of Forestry, College of Agriculture and Veterinary Medicine, Nihon University
-
Shibata Daisuke
Mitsui Plant Biotech. Res. Inst.
関連論文
- (292) 生物防除微生物Pythium oligandrumによる誘導抵抗性発現トマトの青枯病菌接種後における網羅的遺伝子発現解析(平成19年度日本植物病理学会大会講演要旨)
- (251) 生物防除微生物Pythium oligandrumおよびプラントアクティベーター処理トマトにおけるBeta-cyanoalanine synthase遺伝子の発現と誘導抵抗性マーカーとしての利用(平成18年度日本植物病理学会大会講演要旨)
- (372) 生物防除微生物Pythium oligandrum (PO)およびPOの細胞壁タンパク質エリシターによる誘導抵抗性の分子機構解明(2) : 細胞壁由来エリシター処理マイクロトムにおける遺伝子発現変動の網羅的解析(平成17年度日本植物病理学会大会講演要旨)
- P11-1 ミヤコグサにおけるイオンストレス応答遺伝子の比較トランスクリプトーム解析(ポスター紹介,11.植物の有害元素,2009年度京都大会)
- DNAマクロアレイを用いたトマト果実における成熟関連遺伝子の網羅的発現解析
- (301)キュウリモザイクウイルス抵抗性シロイヌナズナにおけるNBS-LRR抵抗性遺伝子ファミリーの発現解析(平成16年度日本植物病理学会大会講演要旨)
- 11-3 シロイヌナズナSTOP1転写因子が制御する遺伝子群によるアルミニウム及び低pH耐性機構の解析(11.植物の有害元素,2009年度京都大会)
- Suppression of carotenoid synthesis in transgenic Arabidopsis cultured cells over-expressing the AHL29/SOB3 gene
- 2Fp07 FT-ICR/MS基質探索法を利用したBacillus由来P450酸化酵素の機能解析(酵素学,酵素工学,タンパク質工学,一般講演)
- 1G11-1 FT-ICR MSを利用した酸化酵素活性の一斉スクリーニング法の開発(酵素学,酵素工学,タンパク質工学,一般講演)
- An approach to peak detection in GC-MS chromatograms and application of KNApSAcK database in prediction of candidate metabolites
- 2Ep24 トランスポリイソプレン生合成のトランスクリプトーム解析(植物細胞工学・組織培養・育種工学,一般講演)
- Transient increase in salicylic acid and its glucose conjugates after wounding in Arabidopsis leaves
- CELヌクレアーゼを用いたトマト系統間のDNA多型の探索
- 矮性ミニトマト品種'マイクロトム'の塩ストレス処理果実における糖・アミノ酸蓄積に関する研究
- トマト果実におけるカルコンとフラバノンの代謝物プロファイリング
- 2P-2100 次世代シークエンサーによる油田微生物のマイクロビオーム解析(9a 生体情報工学,バイオインフォマティクス,一般演題,バイオ情報,伝統の技と先端科学技術の融合)
- P-551 FUNCTIONS OF ARABIDOPSIS CAldOMT
- PHOTO-INDUCTION OF ELECTRON DONATION TO THE INTERSYSTEM CHAIN MEDIATED BY NADPH DEHYDROGENASE IN CYANOBACTERIUM SYNECHOCYSTIS PCC 6803
- ELECTRON TRANSPORT IN Synechococcus sp.PCC 6803 WILD TYPE AND ndhB - DELETED MUTANT UNDER SALT STRESS CONDITIONS
- RESPONCES TO SALT STRESS OF Synechocystis sp. PCC 6803 WILD TYPE AND ndhB-DELETED MUTANT
- Functional Analysis of Isoforms of NADPH:Protochlorophyllide Oxidoreductase (POR), PORB and PORC, in Arabidopsis thaliana
- (1) "マイクロトム"トマト(ナス科モデル植物)の各種植物病原体に対する応答(平成17年度日本植物病理学会大会講演要旨)
- Partial Amino Acid Sequences of Soybean Lipoxygenase L-6 Isolated from Cotyledons
- ANALYSIS OF TEMPERATURE SENSITIVE CELL WALL MUTANTS OF Arabidopsis thaliana
- BETAINE ALDEHYDE DEHYDROGENASE IN MANGROVES
- An Arabidopsis Sigma Factor (SIG2)-Dependent Expression of Plastid-Encoded tRNAs in Chloroplasts
- MOLECULAR MECHANISMS OF THE CHLOROPLAST DIVISION AND DEVELOPMENT IN ARABIDOPSIS THALIANA
- ANALYSIS OF COMPONENTS OF THE PLASTID PROTEIN IMPORT APPARATUS IN ARABIDOPSIS
- Increase of Foreign Gene Expression in Monocot and Dicot Cells by an Intron in the 5' Untranslated Region of a Soybean Phosphoenolpyruvate Carboxylase Gene
- Overexpression of DnaK chaperone from a halotolerant cyanobacterium Aphanothece halophytica increases seed yield in rice and tobacco
- Role of the Putative Membrane-Bound Endo-1,4-β-Glucanase KORRIGAN in Cell Elongation and Cellulose Synthesis in Arabidopsis thaliana
- CELL WALL ANALYSIS OF TEMPERATURE SENSITIVE MUTANT acwl OF A. thaliana
- Regional Insertional Mutagenesis of Specific Genes on the CIC5F11/CIC2B9 Locus of Arabidopsis thaliana Chromosome 5 Using the Ac/Ds Transposon in Combination with the cDNA Scanning Method
- OSCILLATION OF POST-ILLUMINATION INCREASE IN CHL FLUORESCENCE IN CYANOBACTERIA Synechocystis PCC6803
- Production of Arabidopsis insertion mutants using Ac/Ds transposon and characterization of Ds-tagged genes
- CLONING OF THE CYTOKININ-HYPERSENSITIVE 1 GENE, A NEGATIVE REGULATOR OF CYTOKININ SIGNALING
- Root specific expression of Na^+/H^+ antiporter gene from Synechocystis sp. PCC 6803 confers salt tolerance of tobacco plant
- HEAT TOLERANCE OF TOBACCO PLANTS TRANSFORMED WITH DnaK FROM A HALOTOLERANT CYANOBACTERIUM Aphanothece halophytica
- ISOLATION OF Na^+/H^+ ANTIPORTER FROM A HALOTOLERANT CYANOBACTERIUM Aphanothece halophytica
- MOLECULAR CHARACTERIZATION OF BETAINE ALDEHYDE DEHYDROGENASE IN BETAINE ACCUMULATING MANGROVES
- EFFECTS OF COEXPRESSION OF E. coli katE AND bet GENES ON THE TOLERANCE TO SALT STRESS IN A FRESHWATER CYANOBACTERIUM Synechococcus sp. PCC 7942
- GENETIC ENGINEERING OF ABIOTIC TOLERANCE IN PLANTS
- TOLERANCE TO THE SALTS AND TEMPERATURE IN TRANSGENIC RICE PLANT THAT HAS INCREASED PHOTORESPIRATION CAPACITY
- HEAT TOLERANCE OF TOBACCO PLANTS WHICH WERE TRANSFORMED WITH DnaK FROM HALOTOLERANT
- Photosynthetic Adaptation to Salt Stress in Three-Color Leaves of a C_4 Plant Amaranthus tricolor
- Expression of nhaAv Gene Encoding Na^+/H^+ Antiporter from Vibrio alginolyticus in a Freshwater Cyanobacterium Synechococcus sp.PCC 7942 Confers Lithium Tolerance, but not Soduim Tolerance
- Salt tolerance in transgenic tobacco plants expressing DnaK from a halotolerant cyanobacterium Aphanothece halophytica
- INDUCTION OF GLYCINEBETAINE ACCUMULATION IN BARLEY BY HEAT STRESS
- STUDY ON ACCUMULATION OF GLYCINEBETAINE AND IONS USING TRANSGENIC RICE OVEREXPRESSING MICROBODY-TYPE OF BARLEY BADH
- ANALYSIS OF AUTOPHAGY IN HIGHER PLANTS
- ANALYSIS OF AUTOPHAGY IN HIGHER PLANTS
- FUNCTIONAL CHARACTERIZATION OF Na^+/H^+ ANTIPORTER from Synechocystis PCC 6803 WHICH IS HOMOLOGOUS TO PLANT, ANIMAL, and BACTERIA ANTIPORTER
- SUBSTRATE SPECIFICITY AND HEAT STABILITY OF SPINACH AND Escherica coli BETAINE ALDEHYDE DEHYDROGENASE
- A COMPARATIVE STUDY ON THE SUBSTRATE SPECIFICITY AND STABILITY BETWEEN SPINACH AND Escherichia coli BETAINE ALDEHYDE DEHYDROGENASE
- EFFECTS OF SALTS AND TEMPERATURE ON THE PROTEIN FOLDING ACTIVITY OF MOLECULAR CHAPERON Dnak FROM A HALOTOLERANT CYANOBACTERIUM Aphanothece halophytica
- EFFECTS OF OVEREXPRESSION OF Escherichia coli katE AND bet GENES ON THE TOLERNCE TO SALT STRESS IN A FRESHWATER CYANOBACTERIUM Synechococcussp. PCC 7942
- EXPRESSION, PURIFICATION AND KINETIC PROPERTIES OF BETAINE ALDEHYDE DEHYDROGENASE FROM SPINACH AND Escherichia coli
- SYSTEMATIC CONSTRUCTION OF TRANSPOSON-TAGGED LINES THAT HAVE INSERTION MUTATIONS IN REGION-SPECIFIC GENES OF ARABIDOPSIS GENOME
- IDENTIFICATION OF EXPRESSED GENES ON YAC CLONE 5F11 DNA OF ARABIDOPSIS CHROMOSOME 5 BY THE cDNA SELECTION METHOD USING DNA-LATEX PARTICLES AND FUNCTIONAL ANALYSIS OF THE ISOLATED GENES BY TRANSPOSON TAGGING
- The endodermis is essential for the gravitropic responses of hypocotyls and inflorescence stems in Arabidopsis thaliana
- Overexpressin of Mitochondrial Citrate Synthase in Arabidopsis thaliana Improved Growth on a Phosphorus-Limited Soil
- Over Expression of Mitochondrial Citrate Synthase Gene Improves the Growth of Carrot Cells in Al-Phosphate Medium
- Structure and Expression of the Mitochondrial Citrate Synthase Gene in Carrot Cells Utilizing Al-Phosphate
- ANALYSIS OF A CHLOROPLAST MORPHOLOGICAL MUTANT PIE, IN ARABIDOPSIS THALIANA
- Production of T-DNA tag line of Arabidopsis thaliana by an In plant transformation method using activation tagging vector
- The Role of Structural Intersubunit Microheterogeneity in the Regulation of the Activity in Hysteresis of Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase^1
- Characterization of molecular chaperon DnaK from a halotolerant cyanobacterium Aphanothece halophytica for ATPase and protein folding activities under various salinity conditions
- SALT TOLERANCE OF FRESHWATER CYANOBACTERIUM Synechococcus sp. PCC 7942 TRANSFORMED WITH Na^+/H^+ ANTIPORTER GENE FROM Vibrio alginolyticus
- ISOLATION OF cDNA ENCODING METHYL JASMONATE-INDUCED CYTOCHROME P450 FROM SOYBEAN SUSPENSION-CULTURED CELLS
- METHYL JASMONATE-INDUCIBLE GENES IN SOYBEAN SUSPENSION-CULTURED CELLS
- ISOLATION OF METHYL JASMONATE-INDUCEDGENES WITH THE DIFFERENTIAL DISPLAY OF mRNA BY RT-PCR
- Kinetic and Cross-Linking Studies on the Interctions of Negative Patch Mutant Plastorcyanin from Silene pratensis with Photosystem I Complexes from Cyanobacteria, Green Algae, and Plants
- CLONING AND EXPRESSION OF DnaK GENE FROM HALOTOLERANT CYANOBACTERIUM Aphanothece halophytica
- ROLE OF PsaF SUBUNIT ON THE INTERACTIONBETWEEN PLASTOCYANIN AND PSI COMPLEX
- Distribution of Fallover in the Carboxylase Reaction and Fallover-Inducible Sites among Ribulose 1, 5-Bisphosphate Carboxylase/Oxygenases of Photosynthetic Organisms
- Molecular Cloning, Water Channel Activity and Tissue Specific Expression of Two Isoforms of Radish Vacuolar Aquaporin
- PRIMARY STRUCTURE OF PLASMA-MEMBRANE-TYPE AQUAPORIN OF RADISH AND COMPARISON WITH TONOPLAST AQUAPORINS
- A 96-kDa Glycyrrhizin-Binding Protein (gp96) from Soybeans Acts as a Substrate for Casein Kinase II, and Is Highly Related to Lipoxygenase 3
- High-affinity phosphate transporter genes of Arabidopsis thaliana
- OVEREEXPRESSION OF AN Arabidopsis INORGANIC PHOSPHATE TRANSPORTER GENE IN TOBACCO CULTURED CELLS ENHANCES PHOSPHATE UPTAKE
- MOLECULAR ANALYSIS OF INORGANIC PHOSPHATE TRANSPORTER FROM Arabidopsis thaliana
- Increase of Cinnamaldehyde Groups in Lignin of Transgenic Tobacco Plants Carrying an Antisense Gene for Cinnamyl Alcohol Dehydrogenase
- FUNCTIONAL GENOMICS APPROACHES FOR PHOSPHATE DEPENDENT EXPRESSION OF ARABIDOPSIS GENES
- Cinnamyl Alcohol Dehydrogenase from Aralia cordata : Cloning of the cDNA and Expression of the Gene in Lignified Tissues
- Reproduction dynamics of eelgrass (Zostera marina L.) populations in Ise-Mikawa Bay, central Honshu, Japan
- Transgenically Produced Glycinebetaine Protects Ribulose 1, 5-bisphosphate Carboxylase/Oxygenase from Inactivation in Synechococcus sp.PCC7942 under Salt Stress
- EFFECTS OF BETAINE ON THE PHOTO-SYNTHETIC ELECTRON TRANSPORT OF THE TRANSFORMANT-AND SALT TOLERANT-CYANOBACTERIA
- cDNA Analysis of the Genes Increasing Their Expression on Tension wood Formation in Eucalyptus camaldulensis L.
- Analysis of the genes specific in tension wood formation by cDNA sequencing
- Specific cDNAs on Tension Wood Formation in Differentiating Xylem
- Molecular Cloning of cDNAs of the Genes Expressed in Differentiating Xylem of Tension Wood Formation in Eucalyptus camaidulensis L.
- Nucleotide Sequences and Homology Search of cDNAs expressed on the Early Stage of Tension Wood Formation
- CHANGES OF GENE EXPRESSION INVOLVING TENSION WOOD FORMATION
- Site-Directed Mutagenetic Study on the Role of Negative Patches on Silene Plastocyanin in the Interactions with Cytochrome f and Photosystem I
- Expression and Characterizatio of Met92Gln Mutant Plastocyanin from Silene pratensis
- GLYCINE BETAINE SYNTHESIS GENES AND ITS RESPONSES TO SALT STRESS
- Characterization of Molecular Chaperon DnaK from a Halotolerant Cyanobacterium Aphanothece halophytica
- CYCLIC ELECTRON TRANSFER REACTIONS OFPHOTOSYSTEM I UNDER HIGH SALTS CONDITIONS
- Intermediate-temperature proton conductors and their applications to energy and environmental devices