Cloning and Expression of the nifA Gene of Klebsiella oxytoca in K. pneumoniae and Azospirillum lipoferum(Microbiology & Fermentation Industry)

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The nifA gene in the 2.9-Kb SalI fragment from the nif gene cluster of Klebsiella oxytoca NG13, an associative nitrogen fixer in the rice rhizosphere, was cloned into the SalI site of pACYC184 to obtain pNOW7A (6.8Kb, Cm^r, nifA^+), in which the nifA gene was expressed from the constitutive Tc^r gene promoter. A recombinant plasmid, pNT11 (15.7Kb, Su^r, nifA^+), was constructed by ligating pNOW7A and RSF1010 at single EcoRI sites. Constitutive expression of the nifA gene on pNOW7A in K. pneumoniae UNF714 nifA^- and K. oxytoca NG13 caused derepression of the nitrogenase activity in the presence of NH^+_4. pNT11 induced nitrogenase activity in Azospirillum lipoferum in the presence of NH^+_4 to the level of 0.7% of that in the absence of NH^+_4.
社団法人日本農芸化学会の論文
1986-06-23