Rapid and Sensitive Method for Measuring the Plasma Concentration of Doxorubicin and Its Metabolites
スポンサーリンク
概要
- 論文の詳細を見る
Doxorubicin is an anti-cancer drug with a wide therapeutic range. However, it and its metabolites cause severe side effects, limiting its clinical use. Therefore, measuring the plasma concentration of doxorubicin and its metabolites is important to study the dosing regimen of doxorubicin. We developed a rapid and sensitive method by ultra-high-performance liquid chromatography with fluorescent detection for measuring the plasma concentration of doxorubicin and its metabolites in small volumes (around 10 μL), enabling repeated measurements from the same mouse. The sensitivity of 7-deoxydoxorubicinolone, a major metabolite of doxorubicin, increased about 5 times than those ever reported using conventional HPLC, and the run time was within 3 min. The area under the curve (AUC0—24 h) of doxorubicin was 5.9 μg h/mL similar to the value of 4.16 μg h/mL obtained previously using a conventional HPLC method. This method would provide information that could be used to refine the therapeutic approach to doxorubicin use.
- 公益社団法人 日本薬学会の論文
著者
-
Okuda Haruhiro
Division Of Organic Chemistry National Institute Of Health Sciences
-
Kawanishi Toru
National Institute Of Health Sciences
-
Sakai-Kato Kumiko
Division of Drugs, National Institute of Health Sciences
-
Nanjo Kunie
Division of Drugs, National Institute of Health Sciences
-
Okuda Haruhiro
Division of Drugs, National Institute of Health Sciences
関連論文
- Involvement of Electron Transfer in the Radical-scavenging Reaction of Resveratrol
- Computational Study on Secondary Structure of Oligopeptides Containing α,α-Disubstituted α-Amino Acids
- Computational Study on Helical Structures of Oligopeptides Containing Chiral Cyclic α,α-Disubstituted α-Amino Acids
- Computational Study on Conformation of Oligopeptides Containing Chiral Cyclic α,α-Disubstituted α-Amino Acids
- Computational Study on Conformation of Oligopeptides Controlled by Side Chain Chiralities of α-Amino Acids
- Prediction of Helical Screw Sense of Oligopeptides Containing Chiral Amino Acids : Computational Study
- EPR Study on Stable Magnesium Complexes of the Phenoxyl Radicals Derived from a Vitamin E Model and Its Deuterated Derivatives
- Gene expression profiles of hepatotoxin-treated human hepatocytes can be used to cluster unknown compounds according to their mode of action(Toxicogenomics, Toxicoproteomics, Proceedings of the 32nd Annual Meeting)
- Prediction of Helical Screw Sense of Oligopeptides Containing Chiral α, α-Disubstituted Amino Acids
- STUDIES ON NITROSAMINE FORMATION BY THE INTERACTION BETWEEN DRUGS AND NITRITE. I. : Measurement of the amount of nitrosamine formed in rat and guinea pig stomachs.
- Present State of New Chiral Drug Development and Review in Japan
- Feasibility of ^F-NMR for Assessing the Molecular Mobility of Flufenamic Acid in Solid Dispersions
- Participation of Cofilin in Opsonized Zymosan-Triggered Activation of Neutrophil-Like HL-60 Cells through Rapid Dephosphorylation and Translocation to Plasma Membranes^1
- Computational Study on Helical Structure of α,α-Disubstituted Oligopeptides Containing Chiral α-Amino Acids
- LOSS OF VIABILITY AFTER DISULFIRAM TREATMENT WITHOUT PRECEDING DEPLETION OF INTRACELLULAR GSH
- STUDIES ON NITROSAMINE FORMATION BY THE INTERACTION BETWEEN DRUGS AND NITRITE. II. : Hepatotoxicity by the simultaneous administration of several drugs and nitrite.
- Freeze-Drying of Proteins in Glass Solids Formed by Basic Amino Acids and Dicarboxylic Acids
- Stabilization of Protein Structure in Freeze-Dried Amorphous Organic Acid Buffer Salts
- Glass-State Amorphous Salt Solids Formed by Freeze-Drying of Amines and Hydroxy Carboxylic Acids : Effect of Hydrogen-Bonding and Electrostatic Interactions
- Integration of Biomolecules into Analytical Systems by Means of Silica Sol-Gel Technology
- Time-Dependent Changes of Oxytocin Using 1H-NMR Coupled with Multivariate Analysis: a New Approach for Quality Evaluation of Protein/Peptide Biologic Drugs
- Design of Short α-Helical Peptides for Transcriptional Inhibitor of Nuclear Receptor
- Computational Study on Conformation of Oligopeptides Containing α,α-Disubstituted Amino Acids
- Conformational Studies of Diastereomeric -Leu-Aib- Peptides
- Design of Stapled Helical Peptides for Catalytic Enantioselective Epoxidation of α,β-Unsaturated Ketones
- Synthesis and Enhanced Radical Scavenging Activity of a Conformationally Constrained Epigallocatechin Analogue
- Comparison of Particle Size and Dispersion State among Commercial Cyclosporine Formulations and Their Effects on Pharmacokinetics in Rats
- Alterations in the Detergent-Induced Membrane Permeability and Solubilization of Saturated Phosphatidylcholine/Cholesterol Liposomes: Effects of Poly(ethylene glycol)-Conjugated Lipid
- Polyethylene Glycol Prevents in Vitro Aggregation of Slightly Negatively-Charged Liposomes Induced by Heparin in the Presence of Bivalent Ions
- Rapid and Sensitive Method for Measuring the Plasma Concentration of Doxorubicin and Its Metabolites
- Component Crystallization and Physical Collapse during Freeze-Drying of L-Arginine–Citric Acid Mixtures
- Freeze-Drying of Proteins in Glass Solids Formed by Basic Amino Acids and Dicarboxylic Acids
- A New Approach to Characterization of Insulin Derived from Different Species Using 1H-NMR Coupled with Multivariate Analysis