Isolation of Mutants of the Nitrogen-Fixing Actinomycete Frankia
スポンサーリンク
概要
- 論文の詳細を見る
Frankia is a nitrogen (N)-fixing multicellular actinomycete which establishes root-nodule symbiosis with actinorhizal plants. Several aspects of Frankia N fixation and symbiosis are distinct, but genes involved in the specific features are largely unknown because of the lack of an efficient mutant screening method. In this study, we isolated mutants of Frankia sp. strain CcI3 using hyphae fragments mutagenized by chemical mutagens. Firstly, we isolated uracil auxotrophs as gain-of-function mutants resistant to 5-fluoroorotic acid (5-FOA). We obtained seven 5-FOA resistant mutants, all of which required uracil for growth. Five strains carried a frame shift mutation in orotidine-5'-phosphate decarboxylase gene and two carried an amino acid substitution in the orotate phosphoribosyltransferase gene. Secondly, we isolated mutants showing loss-of-function phenotypes. Mutagenized hyphae were fragmented by ultrasound and allowed to multiply at their tips. Hyphae were fragmented again and short fragments were enriched by filtration through 5 μm pores filters. Next-generation and Sanger sequencing revealed that colonies formed from the short hyphae fragments consisted of cells with an identical genotype. From the mutagenized colony population, we isolated three pigmentation mutants and a mutant with reduced N-fixation activity. These results indicate that our procedure is useful for the isolation of loss-of-function mutants using hyphae of Frankia.
- 日本微生物生態学会の論文
著者
-
YAMAURA MASATOSHI
Graduate School of Science and Engineering, Kagoshima University
-
ABE MIKIKO
Graduate School of Science and Engineering, Kagoshima University
-
Kucho Ken-ichi
Graduate School Of Science And Engineering Kagoshima University
-
Uchiumi Toshiki
Graduate School Of Sci. And Engineering Kagoshima Univ.
-
Kakoi Kentaro
Graduate School Of Science And Engineering Kagoshima University
-
Yamaura Masatoshi
Graduate School Of Science And Engineering Kagoshima University
-
Kamiharai Toshihito
Graduate School of Science and Engineering, Kagoshima University
-
Tamari Daiki
Department of Chemistry and Bioscience, Faculty of Science, Kagoshima University
関連論文
- ウツボカズラの補虫器より分泌される糖質と昆虫誘導効果
- Transient Transformation of Frankia by Fusion Marker Genes in Liquid Culture
- モデルマメ科植物ミヤコグサのスプリットルート法を用いた根粒形成のオートレギュレーションに関する研究
- 8-12 ダイズ根粒シンビオソーム溶液を処理した単生ダイズ根粒菌に誘導される新規遺伝子応答の網羅的解析(8. 共生, 2006年度秋田大会講演要旨)
- 6-8 ダイズ根粒菌のゲノムの構造比較と共生窒素固定能(6. 土壌生物, 2006年度秋田大会講演要旨)
- B-08 マクロアレイによるダイズ根粒菌バクテロイドの網羅的遺伝子発現解析(遺伝子伝播,口頭発表)
- A-12 マクロアレイによるダイズ根粒菌株のゲノム比較 : Bradyrhizobium属細菌のゲノム進化と多様性(分類・系統解析,口頭発表)
- マクロアレイによるダイズ根粒菌株のゲノム比較(6. 土壌生物, 2004年度大会講演要旨集)
- 27-C-21 炭素源飢餓条件下で発現誘導されるミヤコグサ根粒菌の遺伝子(生理・増殖,一般講演)
- 8-17 共生・微好気・炭素飢餓条件におけるミヤコグサ根粒菌の網羅的遺伝子発現解析(8.共生)
- B-06 炭素飢餓条件下におけるミヤコグサ根粒菌の網羅的な遺伝子発現解析(生理・増殖2,口頭発表)
- 6-21 全ゲノム塩基配列情報に基づくミヤコグサ根粒菌の網羅的遺伝子発現解析(6.土壌生物)
- PB-33 Yam bean (Pachyrhizus erosus(L.) Urban)と共存する細菌叢(共生/相互作用,ポスターセッションB,ポスター発表)
- S1-3 熱帯産ダイズ根粒より分離したバクテリアのpHストレス抵抗性と根粒形成遺伝子の解析(シンポジウム1 酸性環境の微生物生態)
- Enhanced symbiotic nitrogen fixation by Lotus japonicus containing an antisense β-1,3-glucanase gene
- Expression analysis of nitric oxide synthesis related gene in Lotus japonicus
- Symbiotic Rhizobium and Nitric Oxide Induce Gene Expression of Non-symbiotic Hemoglobin in Lotus japonicus
- Control of Nodule Number by the Phytohormone Abscisic Acid in the Roots of Two Leguminous Species
- Isolation of Endophytic Frankia from Root Nodules of Casuarina equlsetifolia and Infectivity of the Isolate to Host Plants (Soil Biology)
- 共生窒素固定研究の最新情報(9)再び植物と根粒菌の相互関係
- Expression of Symbiotic and Nonsymbiotic Globin Genes Responding to Microsymbionts on Lotus japonicus
- ANALYSIS OF NODULE FORMING ABILITY ON TrEnodDR1 TRANSGENIC CLOVER
- EXPRESSION OF SYMBIOTIC AND NONSYMBIOTIC GLOBIN GENES RESPOND TO THE MICROSYMBIONT INFECTION ON LOTUS JAPONICUS
- Genome and Chromosome Dimensions of Lotus japonicus
- 熱帯性マメ科樹木モルッカネム(Paraserianthes falcataria(L. )FOSBERG)に共生する根粒菌の系統解析
- 222 Paraserianthes falcatariaから分離された根粒菌の多様性
- 熱帯産木本マメ科植物Paraserianthes falcatariaの根粒より分離した菌の特性解析
- マメ科植物染色体上でのレグヘモグロビン遺伝子の直接検出
- ユリ属 Lilium L. 雑種のAFLP分析
- STRUCTURE AND EXPRESSION OF GLOBIN GENES IN LOTUS JAPONICUS
- ANALYSIS OF GENOMIC LEGHEMOGLOBIN GENES OF LOTUS JAPONICUS
- Detection of the Leghemoglobin Gene on Two Chromosomes of Phaseolus vulgaris by in situ PCR Linked-Fluorescent in situ Hybridization (FISH)
- CLONING AND DETECTION OF LEGHEMOGLOBIN GENE ON THE METAPHASE CHROMOSOME OF LOTUS JAPONICUS
- DETECTION OF LEGHEMOGLOBIN GENE ON THE CHROMOSOME OF PHASEOLUS AND VICIA BY FLUORESCENT IN SITU HYBRIDIZATION
- Rehabilitation process of burned tropical forest ecosystem through the symbiotic interaction between legume and Rhizobium : Field investigation in Bukit Bangkirai, East Kalimantan, Indonesia
- MORPHOLOGY AND PROTEIN COMPOSITION OF BACTEROIDS IN INEFFECTIVE NODULES INDUCED QN E135 (sym13) PEA MUTANT
- Up- and down-regulated genes of white clover by the nod genes derived from Rhizobium leguminosarum bv. trifolii strain 4S
- アンチセンスRNA法によるサツマイモの低アミロース化
- Phylogenetic Analysis of Root-Nodule Bacteria Isolated from Yam Bean(Pachyrhizus erosus L. Urban)
- Influence on early step of infection by materials of Rhizobium periplasma
- CLONING OF GBSS GENE FROM SWEET POTATO
- マメ科植物と根粒菌の共生成立初期の分子認識と遺伝子応答
- TRANSFER OF Rhizobium NODULATION GENE TO Agrobacterium FOR EXTENSION OF HOST RANGE
- 低アミロースサツマイモの作出について
- PR-promoterの発動と根粒菌感染との関係
- 根粒菌染色体への溶原性ファージの溶原化機構について
- クローバ根毛細胞の変形におよぼすnod gene発現産物の影響
- EFFICIENT EXPRESSION OF INTEGRATEDCHROMOSOMAL NOD GENES IN RHIZOBIUM LEGUMINOSARUM bv. TRIFOLII
- THE INFLUENCE OF NOD METABOLITE ON ROOTHAIR CURLING AND DEFORMATION
- Analysis of Feeding Mechanism in a Pitcher of Nepenthes hybrida
- General Characterizations of Two Aeromonas sobria Strains Isolated from Sillago parvisquamis
- Occurrence of Vibrionaceae Bacteria in Five Kind Species of Marine Fish Organs in Southern Area of Japan
- Characteristics of Bacteroids in Indeterminate Nodules of the Leguminous Tree Leucaena glauca
- Characteristics of Bacteroids in Indeterminate Nodules of the Leguminous Tree Leucaena glauca
- PHYBシグナリングを介した光の質と量による根粒形成制御機構
- Nitric Oxide Production Induced in Roots of Lotus japonicus by Lipopolysaccharide from Mesorhizobium loti
- Bradyrhizobium japonicum DNAマクロアレイによる根粒形成、非根粒形成Bradyrhizobiaceae科細菌のゲノム比較(2006年度大会一般講演要旨)
- Involvement of a Novel Genistein-Inducible Multidrug Efflux Pump of Bradyrhizobium japonicum Early in the Interaction with Glycine max (L.) Merr
- Isolation of Mutants of the Nitrogen-Fixing Actinomycete Frankia