Protein Phosphorylation in the Sieve Tubes of Rice Plants

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概要

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• Proteins and their phosphorylation were examined in rice phloem sap that had been collected by the insect laser technique. Analysis by SDS-PAGE indicated that rice phloem sap contained over 150 proteins. The total protein concentration was 150-200 ng μl^<-1>. Analysis of proteins extracted from leaves, roots and seeds revealed that several major low-molecular-weight proteins were confined to the rice phloem sap. Maintenance of rice plants under stable environmental conditions was associated with a constant complement of proteins in the phloem sap. Phosphorylation and dephosphorylation of proteins were detected in the sieve tubes and occurred in a light dependent manner when [γ-^<32>P]ATP was supplied to leaf blades. In darkness, 17-kDa and 24-kDa proteins were phosphorylated. When plants were transferred to the light, 17-kDa and 21-kDa proteins were phosphorylated. Proteins in the rice phloem sap were also phosphorylated in vitro. The phosphorylation was Ca^<2+>_dependent and was inhibited by a protein kinase inhibitor. These results indicate that a signal-transport system that involves phosphorylation of proteins may exist in rice sieve tubes.

• 日本植物生理学会の論文

著者

• 秋山 伸一

  Department Of Biological Production Faculty Of Bioresource Sciences Akita Prefectural University

• HAYASHI Hiroaki

  Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U

• Hayashi Hiroaki

  Department Of Biological Production Faculty Of Bioresource Sciences Akita Prefectural University

• NAKAMURA Shin-ichi

  Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University

• CHINO Mitsuo

  Department of Biological Production, Faculty of Bioresource Sciences, Akita Prefectural University

• MORI Satoshi

  Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Sciences,

• 秋山 伸一

  鹿児島大学医学部附属腫瘍研究施設

• 秋山 伸一

  鹿児島大学腫瘍研

• Hayashi H

  Department Of Biological Production Faculty Of Bioresource Sciences Akita Prefectural University

• Chino Mitsuo

  Department Of Biological Production Faculty Of Bioresource Sciences Akita Prefectural University

• Mori S

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Chino Mitsuo

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Mori Satoshi

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Hayashi Hiroaki

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• Mori S

  Univ. Tokyo Tokyo Jpn

• Mori S

  Meijo Univ. Aichi Jpn

• Nakamura Shin-ichi

  Department Of Applied Physics Faculty Of Scince Tokyo Institute Of Technology

• Hayashi Hiroaki

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Science The Univ

• Hayashi Hiroaki

  Department Of Agricultural Cheinistry Faculty Of Agriculture University Of Tokyo

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