Protoplasts of Pyricularia oryzae P2: Preparation and Regeneration into Hyphal Form
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概要
- 論文の詳細を見る
Protoplasts of Pyricularia oryzae P2, a rice blast mold, were prepared in high yield from the young mycelium of the fungus using lytic enzymes from Bacillus circulans WL12. The majority of the protoplasts had one nucleus per cell. The protoplasts formed a cell wall and eventually reverted to normal mycelial form in liquid medium. The process of regeneration was studied under phasecontrast and electron microscopes. The protoplast built a very thick wall prior to the protrusion of a germ-tube like hypha. Golgi apparatus-like structures appeared in the early stage of regeneration and disappeared later. Electron-transparent amorphous structures accumulated during regeneration. Lomasomes were observed in the regenerated cell walls.
- 社団法人 日本農芸化学会の論文
著者
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Tanaka Hirosato
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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Ogasawara Nagahiro
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata Unirersity
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KAMIMIYA Shuwsei
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
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