Preparation and Properties of β-Glucan Synthase of Pyricularia oryzae P_2(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
A particulate enzyme fraction capable of catalyzing the transfer of glucose from UDP-glucose to β-glucan was prepared from the mycelium and protoplasts of Pyricularia oryzae P_2. An assay method for the β-glucan synthase was developed. About 80% of the β-glucan synthase activity was found in the pellet obtained on centrifugation at 28,000×g for 30 min. The particulate enzyme preparation showed β-glucan and glycogen synthase activities, in a ratio of 7 : 3. The optimum temperature and pH of the enzyme were found to be 20℃and 7.0, respectively. The glucan synthase activity increased 5.7-fold in 5 hr after the onset of protoplast regeneration.
- 社団法人日本農芸化学会の論文
- 1987-03-22
著者
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TANAKA Hirosato
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
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KAMIMIYA Shusei
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
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Kamimiya Shusei
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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Tanaka Hirosato
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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KOMINATO Masayuki
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
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KAMIMIYA Shuwsei
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
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Kamimiya S
Niigata Univ. Niigata Jpn
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Kominato Masayuki
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
関連論文
- Purification and Some Properties of Chitinase B1 from Bacillus circulans WL-12
- Expression in Escherichia coli of the Bacillus circulans WL-12 Structural Gene for β-1, 3-Glucanase A(Microbiology & Fermentation Industry)
- Comparative Studies of β-1,3-Glucanase A1 and B of Bacillus circulans WL-12 : Purifications and Enzymatic Properities
- Structure of the Gene Encoding β-1,3-Glucanase B of Bacillus circulans WL-12
- Cloning and Sequencing of chiC Gene of Bacillus circulans WL-12 and Relationship of Its Product to Some Other Chitinases and Chitinase-Like Proteins
- Site-directed Mutagenesis of the Asp-197 and Asp-202 Residues in Chitinase A1 of Bacillus circulans WL-12
- Effects of Fungal Lytic Enzymes and Non-ionic Detergents on the Actions of Some Fungicides against Pyricularia oryzae(Microbiology & Fermentation Industry)
- Preparation and Properties of β-Glucan Synthase of Pyricularia oryzae P_2(Microbiology & Fermentation Industry)
- Protoplasts of Pyricularia oryzae P2: Preparation and Regeneration into Hyphal Form
- Glycogen Granules in Regenerating Protoplasts of Pyricularia oryzae P2
- Cell Walls of Regenerating Protoplasts of Pyricularia oryzae P2
- Effect of Glucosamine on the Growth of Pyricularia oryzae