Structure of the Gene Encoding β-1,3-Glucanase B of Bacillus circulans WL-12
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概要
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β-1,3-Glucanases B (GlcB) and C (GlcC) are the major β-1,3-glucanases of Bacillus circulans WL-12 detected in the culture supernatant grown in β-1,3-glucan-free medium. The gene (glcB) encoding GlcB was cloned into Escherichea coli and its nucleotide sequence was determined. The open reading frame of the glcB gene encodes a polypeptide of 412 aino acid residues. The N-terminal amino acid sequences of GlcB and GlcC purified from B. circulans culture supernatant were determined to be identical to each other and to the deduced sequence downstream of Ala-29. The N-terminal amino acid sequence, isoelectric point and estimated size of the β-1,3-glucanase prodused by E. coli cells cerrying the cloned glcB gene atreed well with those of GlcB of B. circulans WL-12. The N-terminal to central region of GlcB exhibited high sequence similarity to β-1,3-glucanases of alkalophilic Bacillus AG-430,GlcA1 of B. circulans WL-12 and the 87 kDa β-1,3-glucanase H of B. circulans IAM1165. The C-terminal region of GlcB exhibited sequence similarity to the C-terminal regions of XlnA of Streptomyces lividans, β-1,3-glucanases of Oerskovia xanthineolytica and Arthrobacter sp. YCWD3,and yeast lytic protease I of Rarobacter faecitabidus. Biochemical and sequence data strongly suggested that GlcC detected in the culture supernatant of B. circulans WL-12 corresponded to the catalytic domain of GlcB generated by loss of a C-terminal region of GlcB.
- 社団法人日本生物工学会の論文
- 1995-09-25
著者
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WATANABE Takeshi
Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University
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Okada Takeshi
Deparment of Cardiology, Osaka General Medical Center
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Tanaka H
Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University
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NIKAIDOU Naoki
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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Watanabe T
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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TANAKA Hirosato
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
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Okada T
Tsukuba Univ. Ibaraki Jpn
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AIDA KAZUHIKO
Department of Applied Biological Chemistry, Faculty of Agiculture, Niigata University
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AISAKA MAKOTO
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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Nikaidou N
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Nikaidou Naoki
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Tanaka Hirosato
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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Alam M
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Aida Kazuhiko
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Watanabe Takeshi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Watanabe Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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AIDA Kazuhiko
Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine
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