LysR-type Transcriptional Regulator ChiR Is Essential for Production of All Chitinases and a Chitin-Binding Protein, CBP21,in Serratia marcescens 2170
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概要
- 論文の詳細を見る
To identify the genes required for chitinase production by Serratia marcescens 2170,various Tn5 mutants somehow defective in chitinase production were isolated in a previous study. In order to identify the mutated gene in one of the chitinase-deficient mutants, N1,DNA regions flanking the Tn5 insertion were cloned and sequenced. Sequence comparison showed that the mutation occurred in the ORF located between chiB and cbp, which encode chitinase B and chitin-binding protein CBP21,respectively. The ORF encodes a 313-amino acid polypeptide which has significant similarity with various LysR-type transcriptional regulators, and thus the gene was designated chiR. Targeted mutagenesis confirmed that disruption of the chiR gene results in the phenotype of N1. Gel mobility shift assays using partially purified ChiR protein demonstrated that this protein specifically binds to the intergenic region between chiR and cbp. These results strongly suggest that ChiR is a LysR-type transcriptional regulator which is essential for production of all chitinases and CBP21.
- 社団法人日本農芸化学会の論文
- 2001-02-23
著者
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Regue Miguel
Department Of Microbiology And Parasitology Health Science Division Faculty Of Pharmacy University O
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Regue Miguel
Department Of Microbiology And Parasitology Health Sciences Division Faculty Of Pharmacy University
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WATANABE Takeshi
Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University
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Suzuki Kazuharu
Department Of Nutritional Science Tokyo University Of Agriculture
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Suzuki Kazuharu
東京農業大学 農学部栄養科学科
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Suzuki Megumi
Department Of Cardiology Fujita Health University
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Suzuki Megumi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Suzuki M
Yamagata Univ. Yamagata Jpn
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Suzuki K
Univ. Tokyo Tokyo
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Suzuki Kazushi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University:(present Addres
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Suzuki Kazushi
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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Suzuki K
Department Of Nutritional Science Tokyo University Of Agriculture
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UCHIYAMA Taku
Department of Biosystem Science, Graduate School of Science and Technology, Niigata University
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NIKAIDOU Naoki
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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Suzuki Masakatsu
Laboratory Of Animal Products Chemistry
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Watanabe T
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Nikaidou N
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Nikaidou Naoki
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Sakurai K
Department Of Nutritional Science Tokyo University Of Agriculture
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Uchiyama Taku
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Suzuki Kazuharu
Department Of Nutritional Science Faculty Of Applied Bioscience Tokyo University Of Agriculture
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Watanabe Takeshi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Watanabe Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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