Improved Transformation of the Halo-Tolerant Yeast Zygosaccharomyces rouxii by Electroporation
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概要
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To improve the transformation efficiency of Zygosaccharomyces rouxii by electroporation, glycerol was added to the electroporation buffer and the cells were frozen at −80 °C. These alterations drastically increased transformation efficiency, and we found that competent cells can be preserved at −80 °C without decreasing their transformation efficiency for at least 30 d.
著者
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WATANABE Takeshi
Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University
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Suzuki Kazushi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University:(present Addres
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WATANABE Jun
Manufacturing Division, Yamasa Corporation
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UEHARA Kenji
Manufacturing Division, Yamasa Corporation
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MOGI Yoshinobu
Manufacturing Division, Yamasa Corporation
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YAMAZAKI Tatsuo
Manufacturing Division, Yamasa Corporation
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