Purification and Partial Characterization of a Novel Glucanhydrolase from Lipomyces starkeyi KSM 22 and its Use for Inhibition of Insoluble Glucan Formation
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概要
- 論文の詳細を見る
A novel glucanhydrolase from a mutant of Lipomyces starkeyi ATCC 74054 was purified. The single protein (100 kDa) showed either dextranolytic or amylolytic activity. We referred to the glucanhydrolase as a DXAMase. The DXAMase was produced in a starch medium and it was 3.75-fold more active for hydrolysis of the purified insoluble-glucan of Streptococcus mutans than Penicillium funiculosum dextranase. Aggregation of S. mutans cells with dextran and adherence to glass were eliminated by incubating with the DXAMase. The addition of DXAMase (0.1IU/ml) to the mutansucrase reaction digest with sucrose reduced the formation of insoluble-glucan about 80%. Also the DXAMase (0.5 IU/ml) removed 80% of the pre-formed sucrose-dependent adherent film. These in vitro properties of L. starkeyi KSM 22 DXAMase are desirable for its application as a dental plaque control agent.
- 社団法人日本農芸化学会の論文
- 2000-02-23
著者
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千葉 誠哉
Graduate School Of Agriculture Hokkaido University
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木村 淳夫
北海道大学大学院農学研究院応用生命科学部門
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KIMURA Atsuo
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
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CHIBA Seiya
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
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Chiba Seiya
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Kim Doman
Research Institute For Catalysis Chonnam National University:research Center For New-biomaterials In
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Kim Doman
School Of Biological Sciences And Technology And Institute Of Bioindustrial Technology Jeonnam Natio
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Kimura Atsuo
Hokkaido Univ.
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Kimura Atsuo
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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Kimura Atsuo
Graduate School Of Agriculture Hokkaido University
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RYU SuJin
Department of Chemical Engineering Chonnam National University
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RYU HwaJa
Department of Fine Chemical Engineering Chonnam National University
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DAY DonalF.
The Audubon Sugar Instistute, Louisiana State University
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Day Donalf.
The Audubon Sugar Instistute Louisiana State University
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Kimura Atsuo
Hokkaido University
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Ryu Su-Jin
Department of Chemical Engineering Chonnam National University
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Ryu Hwa-Ja
Department of Fine Chemical Engineering Chonnam National University
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