Glutathione Transferase from Mucor javanicus(Biological Chemistry)

概要

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Glutathione transferase was purified from Mucor javanicus and characterized as a dimer of identical subunits (M_r; 22,000). Kinetics and Km values of M. javanicus glutathione transferase, 0.48mM and 1.0mM (at pH 6.5) for glutathione and 1-chloro-2,4-dinitrobenzene, are close to those of rat liver enzyme, but occurrence of isoenzymes was not recognized in M. javanicus. The best substrate of M. javanicus glutathione transferase was 1-chloro-2,4-dinitrobenzene. On the other hand, 2,3-, 2,4-, 2,5-, and 3,4-dichloronitrobenzenes were converted to the related conjugates by 0.17 to 0.35% of the velocity toward the best substrate. This is correlated to the previous result that 2,3-and 2,4-dichloronitrobenzenes were metabolized to 2-methylthio derivatives by the incubation with M. javanicus.
社団法人日本農芸化学会の論文
1988-01-23