Identification of the 4-Hydroxycinnamate Decarboxylase (PAD) Gene of Klebsiella oxytoca
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概要
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A 7.1-kbp DNA fragment isolated from a wild strain of Klebsiella oxytoca was sequenced, leading to the identification of 10 open-reading frames (ORFs), including a 504-bp Pad gene. The Pad gene of the Gram-negative bacterium was subsequently expressed in Escherichia coli as a chimeric Pad. The deduced amino acid (AA) sequence of the Pad gene from wild-type K. oxytoca showed approximately 50% homology to those of other bacterial PADs from Gram-positive bacilli plus a coccus. These data and a genomic library search of some γ-proteobacteria, including E. coli and Vibrio sp., indicated that PAD of K. oxytoca is a member of the bacterial PAD family characteristic of Gram-negative bacteria. Using Pad-specific PCR primers designed from the Gram-negative bacterial Pad of K. oxytoca, Pad genes of two further strains of K. oxytoca, another wild isolate and JCM 1665 and two PAD-positive Enterobacter spp. were successfully amplified for specific Pad detection.
- 日本農芸化学会の論文
- 2008-01-23
著者
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Tahara Satoshi
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University:crest Japan Scienc
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Tahara Satoshi
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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HASHIDOKO Yasuyuki
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
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Hashidoko Yasuyuki
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University
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UCHIYAMA Hirofumi
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
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KURIYAMA Yuhki
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
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Kuriyama Yuhki
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
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Uchiyama Hirofumi
Division Of Applied Bioscience Research Faculty Of Agriculture Hokkaido University
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Tahara Satoshi
Division Of Applaied Bioscience Graduate School Of Agriculture Hokkaido University
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HASHIDOKO Yasuyuki
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
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TAHARA Satoshi
Division of Applied Bioscience, Research Faculty of Agriculture, Hokkaido University
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