Cloning of a DNA Fragment Carrying the 4-Hydroxycinnamate Decarboxylase (pofK)Gene from Klebsiella oxytoca, and Its Constitutive Expression in Escherichia coli JM109 Cells
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概要
- 論文の詳細を見る
The 4-hydroxycinnamate decarboxylase gene (pofK gene) was cloned from Klebsiella oxytoca, an epiphytic bacterium able to decarboxylate hydroxycinnamic acids to styrene derivatives, in Escherichia coli JM109. Colonies of the enzyme activity-positive transformants were screened by a selection assay combined with an antifungal test using Cladosporium herbarum IHU9262 as the bio-indicator. Two positive transformants constitutively producing 4-hydroxycinnamate decarboxylase were obtained. One of the transformants had a recombinant plasmid designated as pTCD100 in which a 9.6kb HindIII segment carrying pofK gene was contained. As the expression of the pofK gene in K. oxytoca was substrate-inducible, it was most likely that the pofK gene expression in E. coli cells was free from any regulation by a pofK gene repressor postulated in K. oxytoca cells. The decarboxylase synthesized in E. coli cells showed almost the same specific activity as that of K. oxytoca induced by the substrate.
- 社団法人日本農芸化学会の論文
- 1994-01-23
著者
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Mizutani J
Hokkaido Univ. Sapporo Jpn
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Mizutani J
Jrdc Plant Ecochemicals Project Eniwa Rbp
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Mizutani Junya
Department Of Applied Bioscience Faculty Of Agriculture Hokkaido University
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Mizutani Junya
Plant Ecochemicals Research Center
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Mizutani Junya
Faculty Of Agriculture Hokkaido University
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Mizutani Junya
Plant Ecochemicals Project Research Development Corporation Of Japan (jrdc)
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Hashidoko Yasuyuki
Jrdc Plant Ecochemicals Project Eniwa Rbp
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Hashidoko Yasuyuki
Jrdc Plant Ecochemicals Project
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Hashidoko Yasuyuki
Division Of Applied Bioscience Graduate School Of Agriculture Hokkaido University:crest Japan Scienc
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Mizutani Junya
Jrdc Plant Ecochemicals Project Eniwa Rbp
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橋床 泰之
Graduate School Of Agriculture Hokkaido University
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Hashidoko Y
Hokkaido Univ. Sapporo Jpn
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URASHIMA Mimako
JRDC Plant Ecochemicals Project
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Urashima M
Jrdc Plant Ecochemicals Project Hokkaido Jpn
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Mizutani J
Plant Ecochemicals Res. Center Eniwa Jpn
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Mizutani Junya
Jrdc Plant Ecochemicals Project Eniwa Rbp Center Building
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Mizutani Junya
Jrdc Mizutani Plant Ecochemicals Project Eniwa Rpb Center Bldg.
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