Viral Genome Delivery into Detached and Intact Leaf Tissues of Vigna unguiculata by RNA-coated Gold Particles Using the Improved Particle Gun.
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概要
- 論文の詳細を見る
Successful delivery of genomic RNA of sweet clover necrotic mosaic virus to detached and intact leaf tissues of Vigna unguiculata cv. California Black Eye, has been demonstrated by using a simple biolistic device equipped with a polyacetal macroprojectile with RNA-coated gold particles, driven by gas pressure from a nitrogen cylinder controlled by a solenoid valve. The device is free of explosive heat and thus cell damage due to delivery operation is minimized.
- 日本学士院の論文
著者
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Hashidoko Yasuyuki
Jrdc Plant Ecochemicals Project
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Mizutani Junya
Jrdc Mizutani Plant Ecochemicals Project Eniwa Rpb Center Bldg.
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Kakuta Hideo
Jrdc Plant Ecochemicals Project Eniwa Rbp Center Building
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HIRUKI Chuji
Department of Agricultural, Food and Nutritional Science, University of Alberta
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HASHIDOKO Yasuyuki
JRDC Plant Ecochemicals Project, Eniwa Research Business Park
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GE Zhongming
Department of Plant Science, University of Alberta
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FIGUEIREDO Gina
Department of Plant Science, University of Alberta
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