DNA Amplifcation Based on Polymerase Chain Reaction for the Sensitive Detection of the Mycoplasmalike Organism Associated with Paulownia Witches'-Broom.
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概要
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DNA amplification by polymerase chain reaction (PCR) was employed to detect the mycoplasma-like organism (MLO) associated with paulownia witches'-broom (PaWB). A 1.2kb DNA fragment of PaWB MLO was amplified with primer pair R16F2R2. A minimum of only 16pg total nucleic acids from infected paulownia tissue culture maintained at 25-28°C was needed to detect PaWB MLO. An appropriate concentration of total nucleic acids for amplification used for detection of PaWB MLO from the tissue culture was demonstrated to be 240pg/μl to 6.5ng/μl. PaWB MLO DNA content in total nucleic acids from MLO-infected tissue culture was as high as 5 times that from MLO-infected paulownia plant grown at 23-25°C in the greenhouse. Results showed that the amplification by PCR is highly effective in detecting the low concentrations of PaWB MLO known to occur in irregular patterns in diseased paulownia plants.
- 日本学士院の論文
著者
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HIRUKI Chuji
Department of Agricultural, Food and Nutritional Science, University of Alberta
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WANG Keri
Department of Agricultural, Food and Nutritional Science, University of Alberta
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KHADHAIR Abdul-Hameed
Department of Agricultural, Food and Nutritional Science, University of Alberta
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KHADHAIR Abdul-Hameed
Department of Plant Science, University of Alberta
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WANG Keri
Department of Plant Science, University of Alberta
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ZHONG Qihong
Department of Plant Science, University of Alberta Edmonton
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