Biolistic Delivery of Foreign DNA or Genomic Transcripts of Plant Virus Full-length cDNA Clones into Monocotyledonous and Dicotyledonous Plant Tissues
スポンサーリンク
概要
- 論文の詳細を見る
Using an improved particle gun driven by compressed nitrogen gas (20kg/cm2 pressure), embryos of oat (Avena sativa) and wheat (Triticum aestivum) or primary leaves of cowpea (Vigna unguiculata) were bombarded with gold particles coated with the vector DNA, pBI121, or genomic transcript RNAs of sweet clover necrotic mosaic virus (SCNMV). GUS gene expression in the oat seedlings was detected in the apical meristems of the primary and emerging lateral roots, and in the root hairs, procambium, stele, coleoptiles, phloem elements, and leaf tissues. However, the retention of the foreign gene in the engineered plants was transient and the gene was lost in the absence of Kanamycin stress. With genomic transcipts of SCNMV full-length cDNA clones of 5ng per bombardment, replication of the delivered genomic viral transcripts was supported by intact primary leaves of cowpea.
著者
-
Hashidoko Yasuyuki
Jrdc Plant Ecochemicals Project
-
Mizutani Junya
Jrdc Mizutani Plant Ecochemicals Project Eniwa Rpb Center Bldg.
-
Kakuta Hideo
Jrdc Plant Ecochemicals Project Eniwa Rbp Center Building
-
HIRUKI Chuji
Department of Agricultural, Food and Nutritional Science, University of Alberta
-
GE Zhongming
Department of Plant Science, University of Alberta
-
FIGUEIREDO Gina
Department of Plant Science, University of Alberta
関連論文
- Efficient Preparation of Browning-Free Glandular Trichome Tissues from the Surface of Leaves of Rosa rugosa Thumb. : STRUCTURE AND FUNCTION OF CELLS : NATURAL PRODUCTS
- Predominant Epiphytic Bacteria on Damaged Polymnia sonchifolia Leaves, and Their Metabolic Properties on Phenolics of Plant Origin
- Cloning of a DNA Fragment Carrying the 4-Hydroxycinnamate Decarboxylase (pofK)Gene from Klebsiella oxytoca, and Its Constitutive Expression in Escherichia coli JM109 Cells
- Decarboxylative Conversion of Hydroxycinnamic Acids by Klebsiella oxytoca and Erwinia uredovora, Epiphytic Bacteria of Polymnia sonchifolia Leaf, Possibly Associated with Formation of Microflora on the Damaged Leaves
- Conversion of (+)-Carotol into (-)-Carota-1,4-dienaldehyde
- Microdialysis for the Analysis of Insect Haemolymph
- Ent-kaurenic Acid and Its Related Compounds from Glandular Trichome Exudate and Leaf Extracts of Polymnia sonchifolia
- Genetic Differentiation of Phytoplasma Isolates Determined by a DNA Heteroduplex Mobility Assay
- Pyromeconic Acid and Its Glucosidic Derivatives from Leaves of Erigeron annuus, and the Siderophile Activity of Pyromeconic Acid
- Rapid detection of a single-base-pair mutation in phytoplasmal conserved genes by DNA heteroduplex mobility assay (HMA)
- Biolistic Delivery of Foreign DNA or Genomic Transcripts of Plant Virus Full-length cDNA Clones into Monocotyledonous and Dicotyledonous Plant Tissues
- Molecular evolution of phytoplasmas based on polymorphisms in the 16S rRNA genes and the 16/23S spacer regions
- Direct Sequencing of Butyrivibrio fibrisolvens 16S rRNA Genes and their 3'- and 5'-Flanking Regions.
- Viral Genome Delivery into Detached and Intact Leaf Tissues of Vigna unguiculata by RNA-coated Gold Particles Using the Improved Particle Gun.
- Molecular Stability of Clover Proliferation Phytoplasma DNA in Periwinkle Plant Tissues after Thermal Treatment under Microwave Conditions.
- PCR (Polymerase Chain Reaction)-based Selection of Phytoplasma-free Clones of Paulownia Tissue Culture after Heat Treatment of Witches'-broom.
- Distribution of Plant Pathogenic Mollicutes in Catharanthus roseus Determined by in situ Molecular Hybridization and DNA Staining with DAPI.
- Chitinase Purified from Alfalfa Infected by Clover Proliferation Mycoplasmalike Organism.
- A comparative study of the sensitivity of dot hybridization assays using cRNA and cDNA for the detection of potato spindle tuber viroid.
- Transmission of sweet clover necrotic mosaic virus.
- The use of cloned DNA probes for diagnosis of noncultivable plant mollicutes.
- DNA Amplifcation Based on Polymerase Chain Reaction for the Sensitive Detection of the Mycoplasmalike Organism Associated with Paulownia Witches'-Broom.